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目的探讨骨髓间充质干细胞(mesenchymal stemcell,MSC)对 T 淋巴细胞杀伤 K562细胞的影响。方法从骨髓中分离培养 MSC,反复贴壁以纯化 MSC,尼龙棉柱法纯化 T 淋巴细胞;将 T 淋巴细胞单独培养基培养或与 MSC共培养3d,流式细胞仪技术检测单独培养或与 MSC 共培养的 T 淋巴细胞亚群的变化;检测其对 K562细胞的杀伤
Objective To investigate the effect of mesenchymal stem cells (MSCs) on killing K562 cells by T lymphocytes. METHODS: MSCs were isolated and cultured from bone marrow. MSCs were purified by repeated adherent method. T lymphocytes were purified by nylon cotton column method. T lymphocytes cultured alone or co-cultured with MSCs for 3 days were detected by flow cytometry Co-cultured T lymphocyte subsets changes; test K562 cell killing