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【目的】柑橘(Citri)是世界上重要的果树。由胶孢炭疽菌[Colletotrichum gloeosporioides(Penz.)]引起的柑橘炭疽病是柑橘生产的主要病害之一。为探索对采后柑橘炭疽病有效的生防措施,分离鉴定柑橘根围土壤中一株细菌T132,并研究其特性及生防效果。【方法】根据菌株T132的形态特征、生理生化特性以及16S rDNA序列对其进行鉴定;通过连续8次在人工培养基上传代培养,测定该菌株的遗传稳定性;采用柑橘果实刺伤挑战接种和拮抗菌液直接浸泡健康果实两种方法研究该菌株对柑橘炭疽病的抑菌防病效果;利用洋葱伯克霍尔德氏菌致病因子的特异性引物检测菌株T132是否为潜在的人类致病菌。【结果】菌株T132鉴定为越南伯克霍尔德氏菌(Burkholderia vietnamiensis)。连续8次在人工培养基上传代培养,菌株T132抑制胶孢炭疽病菌生长的能力没有发生明显改变。菌株T132对胶孢炭疽菌C.gloeosporioides引起的柑橘炭疽病有明显的防治作用,刺伤接种的防效为88.2%,自然发病的防效为54.9%。未检测到该拮抗菌株有人体致病相关的洋葱伯克霍尔德氏菌致病因子(BCESM)毒力基因。【结论】首次报道对柑橘采后炭疽病具有生防效果、对人类相对安全的越南伯克霍尔德氏菌生防菌株。
【Objective】 Citri is the most important fruit tree in the world. Citrus anthracnose caused by Colletotrichum gloeosporioides (Penz.) Is one of the major diseases in citrus production. In order to explore effective biocontrol measures against postharvest citrus anthracnose, a strain of bacteria T132 in citrus rhizosphere soil was isolated and identified and its characteristics and biocontrol effects were studied. 【Method】 The strain T132 was identified by its morphological, physiological and biochemical characteristics and 16S rDNA sequence. The genetic stability of the strain was determined by subculturing eight times on artificial medium. The citrus fruit stabbing challenge challenge and Antagonistic bacteria directly soaked in healthy fruits of two methods to study the bacteriostasis of citrus anthracnose effect of the strain; using Burkholderia cepacia oncogene pathogen specific primers to detect whether the strain T132 is a potential human pathogenic bacteria. 【Result】 Strain T132 was identified as Burkholderia vietnamiensis. Continuous culture on artificial medium for 8 times, the ability of strain T132 to inhibit the growth of Colletotrichum gloeosporioides did not change significantly. Strain T132 had obvious preventive and curative effects on citrus anthracnose caused by C. gloeosporioides, which was 88.2% for stab wounds and 54.9% for natural disease. This antagonistic strain was not detected to have the human pathogenic Burkholderia cepacia virulence gene (BCESM) virulence gene. 【Conclusion】 For the first time, it has been reported that it is biocontrol agent against Burkholderia due to its biocontrol effect on postharvest anthracnose in citrus.