Anti-inflammatory and anti-arthritic effects of Guge Fengtong Formula: in vitro and in vivo studies

来源 :Chinese Journal of Natural Medicines | 被引量 : 0次 | 上传用户:todo158
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Rheumatoid arthritis(RA) is the most common inflammatory arthritis and a major cause of disability. Presently, the clinical therapeutic medicines for inflammatory and arthritic diseases are unsatisfactory due to severe adverse effects or ineffectiveness. The Guge Fengtong formula(GGFT), containing the standardized extracts of Dioscoreae Nipponicae Rhizoma, Spatholobi Caulis, and Zingiberis Rhizoma, has long been used for RA treatment in China. However, the detailed anti-inflammatory and anti-arthritic activity of GGFT has not been reported so far. In the present work, we aimed to evaluate the anti-inflammatory and anti-arthritic effects of GGFT using three in vivo animal models and tried to uncover the underlying mechanism of action in RAW 264.7 macrophages. The obtained results indicated that GGFT significantly attenuated ear edema, decreased carrageenan-induced paw edema, reduced the arthritis score, and reversed the weight loss of the complete Freund’s adjuvant(CFA)-injected rats. Additionally, decrease in synovial inflammatory infiltration and synovial lining hyperplasia in the joints and decline of inflammatory factors(TNF-α and IL-1β) in the serum were observed in the GGFT-treated rats. In lipopolysaccharide-activated RAW264.7 macrophages, GGFT reduced the production of NO, PGE2, and IL-6 and inhibited the expression of i NOS, COX-2, and NF-κB. Our results demonstrated that GGFT possessed considerable anti-inflammatory activity and had potential therapeutic effects on adjuvant induced arthritis in rats, providing experimental evidences for its application in the treatment of RA and other inflammatory diseases. Presently, the clinical therapeutic medicines for inflammatory and arthritic diseases are unsatisfactory due to severe adverse effects or ineffectiveness. The Guge Fengtong formula (GGFT), containing the standardized extracts of Dioscoreae Nipponicae Rhizoma, Spatholobi Caulis, and Zingiberis Rhizoma, has long been used for RA treatment in China. However, the detailed anti-inflammatory and anti-arthritic activity of GGFT has not been reported so far. In the present work, we aimed to evaluate the anti-inflammatory and anti-arthritic effects of GGFT using three in vivo animal models and tried to uncover the underlying mechanism of action in RAW 264.7 macrophages. The obtained results indicated that GGFT significantly attenuated ear edema, decreased carrageenan-induced paw edema, reduced the arthritis score, and the weight loss of the complete Freund’s adjuvant (CFA) -injected rats. A dditionally, decrease in synovial inflammatory infiltration and synovial lining hyperplasia in the joints and decline of inflammatory factors (TNF-α and IL-1β) in the serum were observed in the GGFT-treated rats. In lipopolysaccharide- activated RAW264.7 macrophages, GGFT reduced the production of NO, PGE2, and IL-6 and inhibited the expression of iNOS, COX-2, and NF-κB. Our results characterized that GGFT possessed considerable anti-inflammatory activity and had potential therapeutic effects on adjuvant induced arthritis in rats, providing experimental evidences for its application in the treatment of RA and other inflammatory diseases.
其他文献
距Photokina开幕还有2个多月,虽然这期间没有任何一场有国际知名度的行业展会召开,但各大厂商却也丝毫没有减慢推陈出新的速度。往年,当火热的春季新品高潮过去之后,将进入一
1996年1月,董吉增由地区建委主任调任山东博兴县县委书记。刚上任,他就只身来到县人武部报到,并为人武部党委“一班人”写下了“忠诚于党的武装事业”九个大 In January 19
新型城镇化与农村社会结构的深刻变动密切关联;新型城镇化“新”就“新”在城乡统筹及城乡一体的发展布局中,从根本上消解了传统与现代、城市与乡村的不可通融性;新型城镇化
患儿,女,(3(6/12))岁。因发热、咳嗽、咽痛二天,以咽炎收住。入院后患儿体温波动在38—40℃之间,厌食,乏力,面色苍白无神等中毒症状明显。腹泻,为黄绿色粘液便,量少。腹胀,
这个装置由GGlab工作室设计,在意大利米兰建造完成,并在西班牙瓦伦西亚瓷砖及卫浴展上展出。设计者认为如今的全球社会在公共领域与私人领域之间、室内空间与室外空间之间,需要更大的连续性,从而产生新的协同和更有活力的城市空间,促成一个城市与其居民间更好的关系。  装置名为“Fluldity”,即流动性,用特色陶瓷制品创造双层表皮的城市特色元素。装置外侧由一系列大小变化的空气净化表皮组成,连接着密布排列
位居英超联赛射手榜榜首的桑德兰队球星凯文·菲利普斯,近来可谓春风得意马蹄疾,在新千年开始之际他虔诚地许下了三个愿望,以期自己的事业能在新世纪有崭新的突破,自此星运
巴塞罗那在收购多名球星受挫后,又抛出更新的一颗绣球,愿出六千万美元收购巴西前锋小罗纳尔多。据称里瓦尔多、路易斯·恩里克在这个赛季结束后注定将离开巴塞罗那,因此俱乐
与国际能源公司终止西气东输协议 壳牌石油发言人表示中国石油终止了于2004年7月与多家国际能源公司签订的《西气东输工程合营框架协议》。当时壳牌、俄罗斯天然气工业股份
小孔镗刀夹日本大一工具公司是特殊刀夹、特殊可转位刀片、特殊成形加工刀具等特殊刀具的专业制造企业。最近,该公司开发了一种“超小型刀夹”,可用于铁削加工小13mm的小孔,现已投放
目前,欧盟正在进行F-气体法规修订,在题为“HFCs的低GWP值替代制冷剂:2020年淘汰HFCs的可行性报告”中明确表示,到2020年,将在20个子行业里完全淘汰HFC,涉及行业包括固定式空