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目的了解云南省鼠类携带汉坦病毒情况及其分子特征。方法在居民区和野外捕鼠,用直接免疫荧光试验检测鼠肺中汉坦病毒抗原,阳性鼠肺用RT-PCR法扩增汉坦病毒汉滩型和汉城型的S基因片段,并进行核苷酸序列测定和分析。结果 2006年9-10月在云南省泸西县共捕获鼠类4属6种267只,其中居民区146只,优势鼠种为黄胸鼠;野外121只,优势种为大绒鼠。居民区鼠类汉坦病毒带病毒率为0.85%(1/117),阳性鼠为黄胸鼠;野外鼠类汉坦病毒带病毒率为6.67%(6/90),阳性鼠为大绒鼠和高山姬鼠。对7份阳性鼠肺作RT-PCR扩增,结果从来自大绒鼠的LX378标本中检测到汉滩型病毒S片段核酸,其序列分析结果显示与Tula汉坦病毒Koziky/5276Ma/94株(AJ223601)核苷酸和氨基酸同源性最高,分别为81%、89.8%,而与汉滩型76-118株、汉城型L99株的核苷酸(氨基酸)同源性分别为73.9%(80.9%)、74.4%(77.9%)。结论首次从我国大绒鼠中检测到类似Tula样汉坦病毒的核酸序列,有关该类汉坦病毒在云南的分布、宿主动物和致病性以及全基因序列特征尚需进一步研究。
Objective To understand the situation and molecular characteristics of hantavirus carried by rodents in Yunnan Province. METHODS: Rat models of Hantavirus antigen in rat lung were detected by direct immunofluorescence assay. S-gene fragments of Hantaan and Han-type Hantavirus strains were amplified by RT-PCR and positive Nucleotide sequence determination and analysis. Results A total of 267 4 genera and 6 species of mammals were collected in Luxi County, Yunnan Province from September to October in 2006, of which 146 were residential areas. The predominant species were Rattus flavipectus; In the residential area, the prevalence of Hantaan virus was 0.85% (1/117), and the positive mice were Rattus flavipectus. The wild rodent Hantavirus with virus rate was 6.67% (6/90) And alpine Apodemus. The results of RT-PCR amplification of seven positive rat lungs revealed that the Hantaan virus S fragment nucleic acid was detected from the LX378 specimen of the tissue culture. The sequence analysis showed that it was highly homologous to the Tula virus of Koziky / 5276Ma / 94 strain AJ223601) had the highest nucleotide and amino acid identities (81% and 89.8%, respectively), whereas the homologies with Hanotype 76-118 and Seoul-type L99 were 73.9% (80.9 %), 74.4% (77.9%). Conclusions Nucleic acid sequences of Tula-like Hantavirus were detected for the first time in our country. The distribution, host animals, pathogenicity and sequence characteristics of Hantavirus in Yunnan need to be further studied.