人类膀胱肿瘤的病因与某些化学物质有关。本实验以 N-丁基-N-(4-羟丁基)亚硝基胺(BBN)为致癌剂,以定量灌胃法和腹腔注射法诱发大鼠膀胱肿瘤。72只雌性 Wistar 大鼠随机分成4组,每组18只。6周灌胃组:BBN180mg 导管灌胃,每周2次,共6周。12周灌胃组:BBN90mg 导管灌胃,每周2次,共12周。腹腔注射组:BBN90mg 腹腔注射,每周2次,共12周。三组实验动物接受致癌剂总剂量均为2160mg。另18只大鼠为对照组。实验过程中,有10只腹腔注射组大鼠死于急性中毒或粘连性肠梗阻。实验进行30周后,处死全部存活大鼠,解剖取材。膀胱纵行剖开,借助4倍放大镜观察粘膜形 The causes of human bladder cancer are related to certain chemicals. In this study, N-butyl-N- (4-hydroxybutyl) nitrosamines (BBN) as a carcinogen, quantitative gavage and intraperitoneal injection of rat bladder cancer. Seventy-two female Wistar rats were randomly divided into 4 groups of 18 rats each. 6-week perfusion group: BBN180mg catheter intragastrically, 2 times a week for a total of 6 weeks. Twelve-week irrigation group: BBN90mg catheter intragastrically, twice a week for a total of 12 weeks. Intraperitoneal injection group: BBN90mg intraperitoneal injection twice a week for a total of 12 weeks. Three groups of experimental animals received the total dose of carcinogens were 2160mg. Another 18 rats as control group. During the experiment, 10 intraperitoneal rats died of acute poisoning or adhesive intestinal obstruction. Thirty weeks after the experiment, all the surviving rats were sacrificed and their anatomy was taken. Bladder longitudinal dissection, with 4 times the magnifying glass to observe mucosal shape