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目的:初步探讨黄芪甲苷对心肌缺血心律失常模型大鼠心肌细胞Ca2+浓度的影响。方法:将心肌缺血再灌注模型大鼠随机分为模型组和假手术组,各组给药7d后开始造模。将Fura-2/AM荧光探针负载于大鼠心肌细胞,采用荧光分光光度计检测Ca2+浓度。结果:模型组心肌细胞内游离Ca2+浓度与假手术组比较,差异有显著性(P<0.01);与黄芪甲苷的低剂量组比较,无显著性差异(P>0.05);与黄芪甲苷的中、高剂量组比较,有显著性差异(P<0.01);黄芪甲苷的中、高剂量组与普罗帕酮组比较,无显著性差异(P>0.05)。结论:黄芪甲苷可降低心肌细胞内游离Ca2+浓度,从而减少心律失常的发生,起到抗心律失常的作用。
Objective: To investigate the effect of Astragaloside Ⅳ on the concentration of Ca2 + in cardiomyocytes of rats with myocardial ischemia and arrhythmia. Methods: Myocardial ischemia / reperfusion model rats were randomly divided into model group and sham operation group. The rats in each group began to model after 7 days. The Fura-2 / AM fluorescent probe was loaded on rat cardiomyocytes, and the concentration of Ca2 + was detected by fluorescence spectrophotometer. Results: Compared with the sham group, the intracellular free Ca2 + concentration in the model group was significantly lower than that in the sham operation group (P <0.01); there was no significant difference between the model group and the low dose Astragaloside group (P> 0.05) (P <0.01). There was no significant difference between the medium and high dose astragaloside groups and the propafenone group (P> 0.05). Conclusion: Astragaloside IV can decrease the concentration of free Ca2 + in cardiomyocytes, thereby reducing the incidence of arrhythmia and playing the role of anti-arrhythmia.