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目的 探讨肺癌早期诊断及随访监测的定量指标。方法 应用HE染色、图像分析技术 ,对正常支气管上皮、鳞化上皮、不典型增生上皮及鳞癌Ⅰ级共 86例支气管粘膜活检组织石蜡切片进行细胞形态定量分析。结果 核面积、核面积差异度、核周长、核周长差异度、核形状因子、核形状因子差异度、核体密度、核浆比值、细胞周长、细胞周长差异度 10项参数随病变阶段的递进呈增加或减少趋势 ;核参数的变化方向反映了癌前病变的发展趋势。结论 支气管粘膜中、重度不典型增生是粘膜从良性向恶性转变的关键阶段 ,细胞形态定量分析对肺鳞癌癌前病变的诊断、监测具有积极意义
Objective To investigate the quantitative indicators of early diagnosis and follow-up monitoring of lung cancer. Methods A total of 86 cases of bronchial mucosa biopsy specimens from normal bronchial epithelium, squamous epithelium, atypical hyperplastic epithelium and squamous cell carcinoma of grade Ⅰ were paraffin embedded in paraffin sections of paraffin sections of HE staining and image analysis. Results 10 parameters of nuclear area, nuclear area difference, perinuclear circumference, perinuclear variation, nuclear shape factor, nuclear shape factor difference, nuclear density, nuclear plasma ratio, cell perimeter, The progression of lesion stage showed an increasing or decreasing trend; the direction of change of nuclear parameters reflected the development trend of precancerous lesion. Conclusions In bronchial mucosa, severe dysplasia is the key stage of mucosal transition from benign to malignant. The quantitative analysis of cell morphology has positive significance for the diagnosis and monitoring of precancerous lesions in lung squamous cell carcinoma