以玉米自交系郑58的茎尖为受体,通过农杆菌介导法将抗草甘膦EPSPS基因转入玉米中,研究以茎尖为受体的农杆菌转化体系的可行性。98株转化苗,经过300 ppm的除草剂(农达)筛选,共获得13株转基因植株,经PCR检测,其中7株表现阳性,转化率达7.14%。初步证明外源基因已经整合到玉米基因组中,以玉米茎尖作为受体的转化系统用于基因转化是可行、高效的。 The stem tips of maize inbred line Zheng 58 were used as recipients. The glyphosate resistant glyphosate EPSPS gene was transferred into maize by Agrobacterium tumefaciens-mediated method. The feasibility of Agrobacterium transformation system with shoot tip as receptor was studied. Thirty-nine transformed seedlings were screened by 300 ppm herbicide (Nongda). Thirteen transgenic plants were screened by PCR. Seven of them were positive, with a conversion rate of 7.14%. Preliminary evidence that exogenous genes have been integrated into the genome of corn, corn shoot tip as a receptor transformation system for gene transformation is feasible and efficient.