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[目的]观察中药槐耳对胃癌细胞株MGC803增殖和凋亡的影响。[方法]以不同浓度的槐耳(4、8、16、32、64mg/ml)分别作用于体外培养的人胃癌细胞MGC803,通过MTT法、划痕实验等检测槐耳对MGC803细胞增殖和迁移的作用,倒置显微镜下观察细胞的形态变化,流式细胞仪检测细胞凋亡情况。[结果]MTT实验显示槐耳可抑制MGC803细胞的生长,呈时间及浓度依赖性;显微镜下可观察到细胞肿胀、破裂、呈坏死状;划痕实验显示,4、8mg/ml槐耳分别作用MGC803细胞,划痕愈合明显慢于对照组,呈剂量依赖关系;流式细胞仪可检测到MGC803细胞凋亡率明显增加,呈时间及浓度依赖性。[结论]槐耳可抑制人胃癌MGC803细胞的迁移,且能通过诱导细胞凋亡的方式有效地抑制人胃癌细胞MGC803的生长。
[Objective] To observe the effect of Huaier on the proliferation and apoptosis of gastric cancer cell line MGC803. [Method] The human gastric cancer cell line MGC803 cultured in vitro was treated with different concentrations of Huaier (4,8,16,32,64mg / ml). The proliferation and migration of MGC803 cells were detected by MTT assay and scratch test The morphological changes of the cells were observed under an inverted microscope, and the apoptosis was detected by flow cytometry. [Result] The results of MTT assay showed that Huaierol could inhibit the growth of MGC803 cells in a time and concentration-dependent manner. Cell swelling, rupture and necrosis were observed under the microscope. Scratch experiments showed that 4 and 8 mg / MGC803 cells, scratch healing significantly slower than the control group, in a dose-dependent manner; flow cytometry MGC803 apoptosis rate was significantly increased in a time-and concentration-dependent manner. [Conclusion] Huaier can inhibit the migration of human gastric cancer cell line MGC803, and can effectively inhibit the growth of human gastric cancer cell line MGC803 by inducing apoptosis.