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目的研究IL-8对肝癌细胞HepG2增殖、迁移的影响,探讨整合素α亚基在调控HepG2细胞迁移中的作用。方法以不同浓度(0~125 ng/ml)的IL-8刺激HepG2细胞,分别采用MTT法检测IL-8对肝癌细胞的增殖作用;细胞划痕损伤模型测定不同处理组在0、4、8、12和24 h各时间点对HepG2细胞水平迁移的影响;Transwell法检测刺激24 h后HepG2细胞纵向迁移能力;免疫荧光检测0、125 ng/ml IL-8刺激HepG2细胞8 h后细胞骨架的变化;Western印迹测定整合素α亚基的表达变化规律。结果与对照组相比,IL-8促进HepG2细胞增殖,但各浓度间无明显差异;细胞划痕损伤实验和Transwell实验均表明IL-8可促进HepG2细胞迁移,且具有浓度依赖性;IL-8诱导HepG2细胞骨架重排,使丝状伪足样凸起数目增多;Western印迹结果显示,IL-8上调整合素α亚基的表达,但各亚基具有浓度性差异。结论 IL-8可通过上调整合素α亚基的表达促进HepG2细胞迁移,各α亚基调控作用可能具有差异性。
Objective To study the effect of IL-8 on the proliferation and migration of HepG2 cells and to explore the role of integrin α subunit in the regulation of HepG2 cell migration. Methods HepG2 cells were stimulated with different concentrations of IL-8 (0 ~ 125 ng / ml). The proliferation of hepatocellular carcinoma cells was detected by MTT assay. , 12 and 24 h at each time point on the level of HepG2 cell migration; Transwell method was used to detect the ability of HepG2 cells to migrate longitudinally 24 h after stimulation; Immunofluorescence was used to detect the cytoskeleton of HepG2 cells stimulated by 0,125 ng / ml IL-8 for 8 h Changes; Western blot determination of integrin α subunit expression changes. Results Compared with the control group, IL-8 promoted the proliferation of HepG2 cells, but there was no significant difference between the concentrations. IL-8 promoted the migration of HepG2 cells in a concentration-dependent manner by cell scratch injury assay and Transwell assay. IL- 8 induced HepG2 cytoskeleton rearrangement, which increased the number of filopodia-like protuberances. Western blotting showed that IL-8 upregulated the expression of integrin α subunit, but the concentration of each subunit was different. Conclusion IL-8 can promote the migration of HepG2 cells by upregulating the expression of integrin α subunit. The regulation of each α subunit may be different.