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目的研究三七总皂苷对HSC-T6细胞增殖的影响。方法将复苏后处于对数生长期的HSC-T6单细胞悬液稀释成浓度为2.0×105个/ml,并以0.1ml/孔接种,置于37℃、5%CO2培养箱内培养,24h后将不同稀释度的三七总皂苷按0.1ml/孔加入培养孔。实验另设培养液空白对照组和细胞对照组。每24h观察细胞形态及其生长状况,实验第4天取细胞上清液,所余细胞以MTT法用酶标仪测定OD值,计算出药物对细胞增殖的抑制率。结果三七总皂苷1/1、1/2、1/4、1/8、1/16、1/32、1/64、1/128倍比稀释浓度时的抑制率分别为83.9%、82%、83%、80.4%、85.3%、79.8%、70.7%、58.9%。1/128以下浓度组的抑制率逐渐降低。结论三七总皂苷对HSC-T6细胞的增殖有明显抑制作用,且有明显的浓度依赖,在128倍稀释浓度时仍有较高抑制率且细胞破坏较轻。
Objective To study the effects of Panax notoginseng saponins on the proliferation of HSC-T6 cells. Methods The single cell suspensions of HSC-T6 in the logarithmic phase after resuscitation were diluted to a concentration of 2.0 × 10 5 cells / ml and inoculated at 0.1 ml / well. The cells were cultured in a 37 ° C., 5% CO 2 incubator for 24 h After different dilutions of Panax notoginseng by 0.1ml / hole added to the culture hole. Experiment set up culture medium blank control group and cell control group. The cell morphology and growth status were observed every 24h. The supernatant was collected on the 4th day of experiment. The remaining cells were assayed by MTT method with the microplate reader to determine the inhibition rate of the drug on cell proliferation. Results Panax notoginseng total saponins 1/1, 1/2, 1/4, 1/8, 1/16, 1/32, 1/64, 1/128 times dilution concentration inhibition rate were 83.9%, 82 %, 83%, 80.4%, 85.3%, 79.8%, 70.7%, 58.9%. The inhibition rate of 1/128 and below groups gradually decreased. Conclusion Panax notoginsenoside has a significant inhibitory effect on the proliferation of HSC-T6 cells in a concentration-dependent manner. At the same concentration of 128-fold dilution, Panax notoginseng saponins still has a higher inhibitory rate and less cell destruction.