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背景与目的:谷胱甘肽与细胞对抗癌药物的解毒作用以及对放射性损伤的保护机制密切相关。本文通过对鼻咽癌患者和健康志愿者外周血血浆可的松水平和全血还原型谷胱甘肽含量的昼夜节律进行研究,从而为鼻咽癌患者进行肿瘤的时间治疗提供参考资料。方法:13名鼻咽癌患者(实验组)和14名健康志愿者(正常对照组)参加本项研究。每位受试者均从中午12点开始抽取外周静脉血4.5mL,每隔4h抽血一次,在24h内共抽血6次。血浆可的松水平的检测采用放射免疫法,全血还原型谷胱甘肽含量则采用高效液相色谱法进行检测。结果:鼻咽癌患者组和正常对照组的血浆可的松水平均呈现出明显的昼夜节律的特征,且节律特性相似。两组的血浆可的松水平的峰值均出现在早晨,而谷值均出现在午夜。不同时间点时,两组的全血还原型谷胱甘肽含量不同,且均具有显著性差异(重复测量的方差分析,F=5.18,P=0.02)。余弦分析表明,鼻咽癌患者组呈现出昼夜节律变化趋势(P=0.06),峰值出现在早晨(05∶02),正常对照组也呈现出明显的昼夜节律的特征,峰值出现在早晨(07∶44±01∶56)(P<0.01)。鼻咽癌患者组外周全血中还原型谷胱甘肽的节律调整均值为(19.60±1.11)nmol/mgprotein,正常对照组为(8.95±0.46)nmol/mgprotein。结论:包括晚期患者在内的鼻咽癌患者仍然具有正常的生物节律。鼻咽癌患者全血中还原型谷胱甘肽水平呈现出昼夜节律变化的趋势,与正常对照组节律特性相似。这为临床上选择恰当的时间对患者进行化疗和放疗提供了有价值的参考资料。
BACKGROUND & OBJECTIVE: Glutathione is closely related to the detoxification of anti-cancer drugs by cells and the protective mechanism of radiation injury. In this paper, nasopharyngeal cancer patients and healthy volunteers plasma levels of cortisone and whole blood reduced glutathione content of the circadian rhythm study, so as to provide the reference for the treatment of patients with nasopharyngeal carcinoma in time. Methods: Thirteen patients with nasopharyngeal carcinoma (experimental group) and 14 healthy volunteers (normal control group) were enrolled in this study. Each subject began taking 4.5 mL of peripheral venous blood from 12:00 noon, drawing blood every 4 hours and 6 times within 24 hours. The level of plasma cortisone was detected by radioimmunoassay, and the content of reduced glutathione in whole blood was detected by high performance liquid chromatography. Results: The plasma cortisol levels of patients with nasopharyngeal carcinoma and normal control group showed obvious circadian rhythms with similar rhythm characteristics. Plasma cortisol peak levels in both groups appeared in the morning, while the troughs occurred at midnight. At different time points, the levels of whole blood reduced glutathione were different in both groups, with significant differences (repeated measures ANOVA, F = 5.18, P = 0.02). Cosine analysis showed that the circadian rhythm of NPC patients showed a trend of circadian rhythm (P = 0.06), the peak appeared in the morning (05:02), and the normal control group also showed the characteristics of the circadian rhythm. The peak appeared in the morning : 44 ± 01:56) (P <0.01). The mean rhythmic adjustment of reduced glutathione in peripheral blood was (19.60 ± 1.11) nmol / mgprotein in nasopharyngeal carcinoma patients and (8.95 ± 0.46) nmol / mgprotein in normal controls. CONCLUSIONS: Nasopharyngeal carcinoma patients, including advanced patients, still have normal circadian rhythms. In patients with nasopharyngeal carcinoma, the level of reduced glutathione showed a trend of circadian rhythm, which was similar to that of the normal control group. This provides a valuable reference for chemotherapy and radiotherapy in patients choosing the right time in clinic.