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目的建立一种同时测定大鼠蛇床子素和欧前胡素的血药浓度的方法。方法采用迪马Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),流动相为:甲醇∶水=70∶30(v/v),流速为1.0 mL.min-1,紫外检测波长为320 nm,米非司酮为内标。取大鼠血样90μL进行血浆样品的处理,测定大鼠血浆中蛇床子素和欧前胡素的色谱行为、标准曲线、提取回收率、精密度和准确度、稳定性等。结果蛇床子素、欧前胡素在大鼠血浆中质量浓度测定方法的线性范围分别为10.94～700.00μg.L-1和15.63～1 000.00μg.L-1,线性关系均良好(r=0.999 8、0.999 9,均P<0.05)。大鼠血浆蛇床子素、欧前胡素最低可定量的质量浓度分别为10.94、15.63μg.L-1(以s/n>4计)。大鼠血浆蛇床子素、欧前胡素低、中、高质量浓度的提取回收率分别为98.96%、95.30%、94.88%和98.23%、98.94%、97.99%,大鼠血浆蛇床子素和欧前胡素低、中、高质量浓度的RSD均<15%。结论建立HPLC法具有检测简便、灵敏度高等优点,可快速、可靠地检测大鼠蛇床子素和欧前胡素的血药浓度。
Objective To establish a method for the simultaneous determination of plasma concentration of osthole and imperatorin in rats. Methods The Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) was used with a mobile phase of methanol: water = 70:30 (v / v) at a flow rate of 1.0 mL.min-1 and a UV detection wavelength of 320 nm , Mifepristone as an internal standard. Blood samples of 90 μL were taken from the rats for plasma sample processing. The chromatographic behavior, standard curve, extraction recovery, precision and accuracy of osthole and imperatorin in rat plasma were determined. Results The linear ranges of osthole and imperatorin in rat plasma were 10.94 ~ 700.00μg.L-1 and 15.63-1000.00μg.L-1, respectively (r = 0.999 8, 0.999 9, all P <0.05). The minimum quantifiable concentrations of osthole and imperatorin in rat plasma were 10.94 and 15.63μg.L-1, respectively, in s / n> 4. The recoveries of rat plasma osthole and imperatorin were 98.96%, 95.30%, 94.88% and 98.23%, 98.94% and 97.99% respectively at low, medium and high concentrations of rat plasma. RSD for low, middle and high concentrations of prevarin was <15%. Conclusion The established HPLC method has the advantages of simple detection and high sensitivity, and can rapidly and reliably detect the concentrations of Osthole and Imperatorin in rats.