Although Pinus sibirica and Pinus koraiensis are resistant to cold or low temperatures in the cold temperate regions of the north hemisphere, the former has a stronger cold resistance. Research has been limited to the comparison of physiological responses of the two species to cold stress. In this study, 5-year-old seedlings of P. sibirica and P. koraiensis were subjected to six temperature treatments, [20 ℃ (control), 0 ℃, - 20 ℃, - 40 ℃,- 60 ℃, and - 80℃], under different stress periods (6, 12, 24, and 48 h). The results showed that differences in each physiological index were significant between P. sibirica and P. koraiensis, except for the permeability of cell membranes, reactive oxygen species, proline and sol-uble proteins. An ANOVA test indicated that there were extreme differences among the temperatures for each index, stress time and temperature × time for most indices. All indices showed a similar trend for P. sibirica and P. koraiensis with decreasing temperature or the extension of stress time. Soluble sugars and proline increased at 0 to- 20 ℃ and then remained unchanged with temperature decline. Other indices showed an increase from 20 to- 20 ℃, stable from - 20 to - 40 ℃ and a decrease from- 40 to - 80 ℃. All the indices increased and then declined along with the prolonged cold stress time, except for the control. From 0 to - 40 ℃, the permeability of cell membranes, relative conductivity, reactive oxygen species and malonaldehyde of P. koraiensis seedlings were higher than in P. sibirica, but superoxide dismutase, peroxidase, catalase activity and soluble sugars, proline, and soluble proteins content emerged as opposite. This study compared the physiological mechanism responses to cold stress between P. sibirica and P. koraiensis to provide the basis for the introduction, distribution, and genetic improvement of these coniferous species.