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目的:了解周围神经损伤后患区皮肤血流灌注的动态变化及其与神经传导功能和轴突溃变状况之间的关系。方法:建立大鼠坐骨神经切断模型,应用激光多普勒血流灌注成像动态分析足底血流变化,分别采用腓肠肌复合肌动作电位(CMAP)检测和免疫组织化学染色分析损伤远侧神经传导功能变化和轴突溃变情况。结果:坐骨神经切断后相应足底皮肤血流灌注急剧增高,高灌注状态在损伤后3 d内恢复到接近正常;神经切断后24 h刺激损伤远侧段神经记录的腓肠肌CMAP波幅减小95%以上;损伤后4 d远侧神经段内绝大部分轴突已溃变清除。结论:大鼠神经切断后患区皮肤血流呈高灌注状态且在损伤后3 d内逐渐恢复到正常水平,这种高灌注状态可能不依赖于远侧段神经冲动传导功能的保持,但与轴突溃变状态相关。
Objective: To investigate the dynamic changes of the skin perfusion and its relationship with nerve conduction function and axonal degeneration after peripheral nerve injury. Methods: The rat model of sciatic nerve transection was established. The changes of the blood flow in the foot were analyzed by laser Doppler perfusion imaging. The changes of nerve conduction in the distal part of the injured nerve were detected by CMAP and immunohistochemical staining. And axonal collapse. Results: After perfusion of sciatic nerve, the perfusion of the corresponding skin of the plantar foot increased sharply, and the hyperperfusion state returned to normal within 3 days after injury. The amplitude of CMAP in the gastrocnemius muscle recorded by the nerve distal to the lesion was decreased by 95% 4 days after injury, most of the axons in the distal nerve segment had been degenerated and cleared. CONCLUSION: The cutaneous blood flow in the affected area is highly perfusion after nerve transection and gradually returns to the normal level within 3 days after injury. This hyperperfusion state may not depend on the preservation of the nerve impulse conduction function in the distal segment. However, Unexplained state related.