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目的:探讨醛固酮合酶(CYP11B2)基因-344C/T的多态性与心房颤动(Af)的关系。方法:研究对象均来自湖北地区汉族人群,包括120例Af患者,120例非Af者。采用成组配比研究,取静脉血,提取基因组DNA,采用聚合酶链反应-限制性酶切片段长度多态性(PCR-RFLP)分析技术对2组人群CYP11B2基因-344C/T的多态性进行分析。结果:CYP11B2-344CT+CC基因型频率在Af组与对照组之间差异有统计学意义(53.4%∶37.5%,P=0.037),等位基因在2组间亦存在同样的趋势(C/T=28.8%∶19.6%,P=0.019)。单因素非条件Logistic回归分析CT+CC基因型频率患Af的危险性高(OR=1.82,95%CI1.02～3.22,P=0.04),排除混杂因素后,CT+CC基因型与人群患Af的风险呈弱相关(OR=1.73,95%CI0.99～3.02,P=0.056);排除混杂因素后,左房内径与人群患Af的风险总是呈显著相关(OR=8.14,95%CI3.43～19.31,P=0.000)。结论:在湖北地区汉族人群中,CYP11B2-344C/T的点突变与Af的发病呈弱相关性,有可能是Af的遗传危险因素,左房内径的增加与Af的发病呈显著相关。
Objective: To investigate the relationship between the polymorphism of aldosterone synthase (CYP11B2) gene -344C / T and atrial fibrillation (Af). Methods: All subjects were from Han nationality in Hubei province, including 120 cases of Af and 120 cases of non-Af. The genomic DNA was extracted from the venous blood in a group proportioning study. The polymorphism of -344C / T of CYP11B2 gene in CYP11B2 gene was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Sex analysis. Results: The frequency of CYP11B2-344CT + CC genotypes was significantly different between Af group and control group (53.4% vs 37.5%, P = 0.037). The same tendency was observed between the two groups (C / T = 28.8%: 19.6%, P = 0.019). Univariate non-conditional Logistic regression analysis of CT + CC genotype frequency of Af risk of high (OR = 1.82,95% CI1.02 ~ 3.22, P = 0.04), excluding confounding factors, CT + CC genotype and population risk (OR = 1.73, 95% CI0.99-3.02, P = 0.056). After excluding confounding factors, there was always a significant correlation between the left atrial diameter and the risk of Af in the population (OR = 8.14, 95% CI 3.43-19.31, P = 0.000). CONCLUSION: In Han nationality in Hubei Province, the point mutation of CYP11B2-344C / T is weakly correlated with the onset of Af, which may be the genetic risk factor of Af. The increase of left atrial diameter is significantly correlated with the onset of Af.