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目的探讨应用高效液相色谱串联质谱法(HPLC-MS/MS)测定血浆中西酞普兰药物浓度的方法。方法设立色谱柱为Agilent-ZORBAX-C8柱;流动相:乙腈-0.1%甲酸水溶液(60:40);流速:0.5 ml/min;柱温:20℃;质谱条件为电喷雾电离源(ESI),以多反应监测(MRM)方式进行检测,用于定量分析的反应离子为m/z 325.1 262.1(西酞普兰)和m/z256.2 67.1(苯海拉明,内标)。结果西酞普兰在0.5～100 ng/ml范围内线性关系良好(r=0.9979);西酞普兰的日内、日间精密度RSD均<10%。结论该方法分析速度快,专属性强,灵敏度高,可作为西酞普兰的血浆药物浓度检测方法。
Objective To investigate the method of HPLC for the determination of citalopram in plasma by high performance liquid chromatography tandem mass spectrometry (HPLC-MS / MS). The column was Agilent-ZORBAX-C8. The mobile phase was acetonitrile-0.1% formic acid aqueous solution (60:40), the flow rate was 0.5 ml / min and the column temperature was 20 ℃. The ESI- (MRM), m / z 325.1 262.1 (citalopram) and m / z 256.2 67.1 (diphenhydramine, internal standard) for the quantitative analysis. Results Citalopram had a good linearity (r = 0.9979) in the range of 0.5-100 ng / ml. The intra-day and inter-day precision of citalopram RSD was <10%. Conclusion The method is rapid, specific and sensitive and can be used as a cetmipram plasma concentration test.