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目的:探讨99m锝-氮-二(N-乙基-N-乙氧基二硫代氨基甲酸盐)[99mTc-N(NOEt)2]用于小鼠鼻咽癌肿瘤模型显像中的可行性。方法:采用正常昆明小鼠进行99mTc-N(NOEt)2生物分布实验。皮下种植CNE细胞制作BALb/c裸鼠鼻咽癌肿瘤模型。采用99mTc-N(NOEt)2与99m锝-六(2-甲氧基-2-异丁基异腈)(99mTc-MIBI)SPECT肿瘤模型显像,通过感兴趣区技术记录不同时间肿瘤/非肿瘤组织显像剂分布比值(T/N),对比评估99mTc-N(NOEt)2与99mTc-MIBI在鼻咽癌肿瘤模型显像中的价值。结果:生物分布结果提示99mTc-N(NOEt)2多分布于肝脏、肾脏、肺、脾脏及心脏。99mTc-N(NOEt)2和99mTc-MIBI显像均可以显示鼻咽癌模型肿瘤。注射显像剂后30min时99mTc-N(NOEt)2与99mTc-MIBI在肿瘤部位的T/N差异无统计学意义。99mTc-N(NOEt)2在鼻咽癌肿瘤的T/N于120min达到最高(3.4±0.4),显著高于99mTc-MIBI(2.5±0.3),并且可以清晰显示肿瘤组织。结论:99mTc-N(NOEt)2显像可用于裸鼠鼻咽癌模型的诊断,注射后120min时肿瘤显像最佳,优于99mTc-MIBI。99mTc-N(NOEt)2有望成为一种替代99mTc-MIBI的鼻咽癌显像剂。
Objective: To investigate the effect of 99mTc-N-bis (N-ethyl-N-ethoxy dithiocarbamate) [99mTc-N (NOEt) 2] feasibility. Methods: Normal Kunming mice were used for 99mTc-N (NOEt) 2 biodistribution experiment. BALB / c nude mice model of nasopharyngeal carcinoma was subcutaneously planted with CNE cells. The 99mTc-N (NOEt) 2 and 99m technetium-hexakis (99mTc-MIBI) SPECT tumor models were used for imaging the tumor / non-tumor tissue (T / N) ratio of 99mTc-N (NOEt) 2 and 99mTc-MIBI in the imaging of nasopharyngeal carcinoma. Results: The results of biodistribution indicated that 99mTc-N (NOEt) 2 was distributed in liver, kidney, lung, spleen and heart. Both 99mTc-N (NOEt) 2 and 99mTc-MIBI imaging can show nasopharyngeal carcinoma. There was no significant difference in T / N of 99mTc-N (NOEt) 2 and 99mTc-MIBI at tumor site 30 min after injecting imaging agent. The T / N of 99mTc-N (NOEt) 2 was the highest in nasopharyngeal carcinoma (3.4 ± 0.4) at 120min, which was significantly higher than that of 99mTc-MIBI (2.5 ± 0.3), and the tumor tissue was clearly visible. Conclusion: 99mTc-N (NOEt) 2 imaging can be used for the diagnosis of nasopharyngeal carcinoma in nude mice. The tumor imaging is the best at 120min after injection, which is better than 99mTc-MIBI. 99mTc-N (NOEt) 2 is expected to become a nasopharyngeal imaging agent that can replace 99mTc-MIBI.