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建立了喹啉铜原药的超声波辅助溶剂提取-反相离子对高效液相色谱法的分析方法。该方法使用XBridgeC18色谱柱和可变波长紫外检测器,并以20mmol/L十二烷基硫酸钠水相体系-乙腈(体积比为60∶40)作流动相;流速为0.6mL/min;检测波长为250nm;保留时间为8.52min;方法的线性相关系数为0.9998,样品平均回收率为99.3%,变异系数在0.05%～0.13%之间,对喹啉铜原药的最低检出限为0.02mg/L。
The analytical method of ultrasonic assisted solvent extraction-reverse phase ion-pair high performance liquid chromatography of quinoline copper was established. The method uses a XBridge C18 column and a variable wavelength UV detector and uses a 20 mmol / L sodium dodecyl sulfate aqueous system - acetonitrile (60:40 by volume) as a mobile phase at a flow rate of 0.6 mL / min. The detection The wavelength was 250nm, the retention time was 8.52min, the linear correlation coefficient of the method was 0.9998, the average recovery of the sample was 99.3%, the coefficient of variation was between 0.05% and 0.13%, the lowest detection limit of quinoline copper was 0.02 mg / L.