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目的探讨肝郁阳痿动物模型制作方法。方法获得性交配经验的36只雄鼠随机分为正常组、模型组及模型治疗组。模型组及模型治疗组大鼠每日悬空倒吊在水池上面,并不断激惹,其中模型治疗组大鼠灌胃给予2ml逍遥散浓缩液,每日两次,其它两组则给予等量生理盐水灌胃。两周后观测雄性大鼠的性活动状况,即雄鼠的骑乘潜伏期和骑乘次数,插入潜伏期和插入次数以及雄鼠的射精潜伏期,并计算雄鼠插入与骑乘次数的比值,同时取大鼠血液作血液流变学指标检测。结果与正常组大鼠相比,模型大鼠精神萎靡,性动机以及性活动能力明显减弱,血液粘度明显增高;而用逍遥散治疗后模型大鼠的性动机、性活动能力以及血液粘度均有显著改善。结论本方法可引起模型大鼠的性动机和性活动能力明显减弱,而具有疏肝解郁作用的逍遥散可改善模型大鼠的性动机以及性活动能力,显示运用此方法可制作理想的肝郁阳痿大鼠模型。
Objective To investigate the method of animal model of liver-yang impotence. Methods Thirty-six male mice with sexual mating experience were randomly divided into normal group, model group and model group. The model group and the model treatment group were suspended daily suspended above the pool and continually provoked. The rats in the model treatment group were treated with intragastric administration of 2ml Xiaoyaosan Concentrate twice daily, and the other two groups were given equal amount of physiology Saline irrigation. Two weeks later, the sexual activity of male rats was observed, that is, the male rats’ riding latency and riding times, the insertion latency and the number of insertions, and the male mice ’s ejaculation latency. The ratio of males’ insertion to riding times was also calculated Blood of rats was measured for hemorheology. Results Compared with the normal rats, the model rats were apathetic, their motivation and sexual activity decreased obviously, and the blood viscosity increased obviously. However, the sexual motivation, sexual activity and blood viscosity of model rats after treatment with Xiaoyao San had Significantly improved. Conclusions This method can significantly reduce the sexual motivation and sexual activity of the model rats, while Xiaoyao San, which has the effect of relieving stagnation of liver qi, can improve the sexual motility and sexual activity of the model rats. It shows that this method can make the ideal liver Yu impotence rat model.