论文部分内容阅读
目的 :寻找胶质母细胞瘤 (GBM) 8号、2 2号染色体上可能存在肿瘤抑制基因的杂合性丢失 (LOH)区域 ,为发现和定位肿瘤抑制基因 (TSG)提供线索和依据。方法 :应用聚合酶链反应 (PCR)方法 ,采用荧光标记的引物和 377型DNA序列自动分析仪 ,分别分析了 2 1例GBM 8号、2 2号染色体上 14个、7个微卫星多态性标记的LOH。结果 :本组病例 8号染色体LOH率为 2 3.8% ,在 16 .3%能提供信息位点检测到LOH。 8p的LOH率明显高于 8q ,分别为 2 3.8% ,14.3% ,8q上所有位点的LOH率都在 15 %以下 ,以 8p2 2～ 2 3上D8S5 5 0和D8S5 49的LOH率较高 ,分别是 2 7.8% ,30 .0 %。染色体 2 2q的LOH率为 47.6 % ,在 30 .0 %可提供信息位点存在LOH。以 2 2 q13.2～ 13.3上D2 2S2 74的LOH率最高 (4 1.2 % )。结论 :8号、2 2号染色体等位基因杂合性丢失可能在GBM的分子发病机制中发挥着重要作用 ,在 8p2 2～ 2 3上D8S5 5 0和D8S5 49位点间区域以及 2 2q13.2～ 13.3上D2 2S2 74位点所在区域可能存在多个与GBM相关的肿瘤抑制基因。
OBJECTIVE: To find out the possible loss of heterozygosity (LOH) region of tumor suppressor genes on chromosome 8 and chromosome 22 of glioblastoma (GBM), and to provide clues and evidences for the discovery and localization of tumor suppressor gene (TSG). Methods: Polymerase chain reaction (PCR) was used to detect the polymorphism of 14 microsatellites and 7 microsatellite polymorphisms on 21 chromosomes of GBM 8 and chromosome 22 using fluorescence-labeled primers and 377 DNA sequence automatic analyzer. Sexually-labeled LOH. Results: The LOH rate of chromosome 8 in this group was 23.8%, and LOH was detected in 16.3% of the cases. The LOH rate of 8p was significantly higher than that of 8q, which was 23.8% and 14.3%, respectively. The LOH rates of all sites on 8q were all below 15%, and the LOH rates of D8S5 50 and D8S5 49 from 8p2 2 to 2 3 were higher , Respectively, 2 7.8%, 30.0%. The LOH rate of chromosome 2 2q was 47.6%, and the presence of LOH at 30.0% of available information sites. The LOH rate of D2 2S2 74 was the highest (4 1.2%) at 2 2 q13.2 ~ 13.3. CONCLUSION: The loss of heterozygosity at allele 8 and chromosome 22 may play an important role in the pathogenesis of GBM. The region between D8S550 and D8S549 on 8p2 2 ~ 2 3 and 2 2q13. There may be multiple tumor suppressor genes associated with GBM in the region of 2 ~ 13.3 on the D2 2S2 74 locus.