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目的探讨舒芬太尼后处理对大鼠肠缺血再灌注时肺组织损伤的影响。方法将40只成年雄性Wistar大鼠,随机分为5组(n=8):假手术组(S组)、肠缺血再灌注组(I/R组)及舒芬太尼1μg/kg、5μg/kg、10μg/kg后处理组(SP1-3组)。采用放射免疫法测定血清及肺组织中肿瘤坏死因子(TNF-α)的含量;免疫组织化学法测定肺组织中NF-κB表达水平。结果与S组相比,其余各组TNF-α含量及NF-κB表达水平升高(P<0.05);与I/R组相比,SP1-3组TNF-α含量及NF-κB表达水平降低(P<0.05);SP1-3组间上述指标比较差异无统计学意义(P>0.05)。结论舒芬太尼后处理减轻大鼠肠缺血再灌注时肺损伤的机制可能与下调NF-κB的表达、降低肺组织炎性反应有关。
Objective To investigate the effect of sufentanil on lung injury induced by intestinal ischemia-reperfusion in rats. Methods 40 adult male Wistar rats were randomly divided into 5 groups (n = 8): sham operation group (S group), intestinal ischemia reperfusion group (I / R group) and sufentanil 1μg / kg, 5μg / kg, 10μg / kg post-treatment group (SP1-3 group). The content of tumor necrosis factor (TNF-α) in serum and lung tissue was determined by radioimmunoassay. The expression of NF-κB in lung tissue was detected by immunohistochemical method. Results The levels of TNF-α and the expression of NF-κB in the other groups were significantly higher than those in the S group (P <0.05). Compared with the I / R group, the levels of TNF-α and the expression of NF- (P <0.05). There was no significant difference in the above indexes between SP1 and 3 groups (P> 0.05). Conclusion The mechanism of sufentanil postconditioning in reducing lung injury induced by intestinal ischemia-reperfusion in rats may be related to the downregulation of NF-κB and the decrease of inflammatory reaction in lung tissue.