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[目的]筛选出一种简便快捷的DNA微量提取方法。[方法]从不同处理甘薯植株的叶片中提取DNA样品并用紫外分光光度法和琼脂糖凝胶电泳法检测其纯度和质量。[结果]正常光照下甘薯植株的DNA产量最高,48 h黑暗处理的次之,24 h黑暗处理的最低。在正常光照下和48 h黑暗处理中顶芽的DNA产量都明显高于叶片;在24 h黑暗处理中,顶芽与叶片的DNA产量比较接近。叶片DNA的纯度高于顶芽,正常光照下和24 h黑暗处理中DNA的纯度高于48 h黑暗处理中的。DNA电泳图谱表明,正常光照下叶片DNA的电泳条带一般比顶芽的好。48 h黑暗处理中顶芽和叶片DNA的电泳条带没有很大差异。[结论]该试验为大批量实生苗DNA的快速提取和多种分子生物学研究奠定了实践基础。
[Objective] The research aimed to screen a simple and quick DNA micro-extraction method. [Method] DNA samples were extracted from leaves of different sweet potato plants and their purity and quality were determined by UV spectrophotometry and agarose gel electrophoresis. [Result] The yield of DNA of sweet potato plants under normal light was highest, followed by darkness at 48 h and lowest at 24 h. Under normal light and 48 h dark treatment, the DNA yield of the top buds was significantly higher than that of the leaves. The DNA yield of the top buds and leaves was close at 24 h darkness. The DNA purity of leaves was higher than that of the top buds. The purity of DNA in darkness was higher than that in 48 h under normal light and 24 h darkness. DNA electrophoresis patterns showed that electrophoresis bands of leaf DNA were generally better than those of terminal buds under normal light. The electrophoretic bands of the top buds and leaf DNA did not differ significantly during the 48 h dark treatment. [Conclusion] This experiment laid the practical foundation for the rapid extraction of large quantities of seedlings DNA and many molecular biological studies.