易位导致上皮性肿瘤的发生、发展,特别在前列腺癌的发展过程中有着十分重要的作用。为了更好的理解融合基因转录体的作用和阐明多灶性肿瘤的克隆组成,作者发明了一种多通道原位检测和识别融合基因转录体表达的技术。与免疫组化相比分为TMPRSS2-ERG融合阴性和融合阳性的前列腺肿瘤。在人类前列腺癌组织中,作者对最普遍存在的TMPRSS2-ERG融合变异体进行了识别和量化,第一次通过原位测序法直接测定了不同基因融合转录体的比例。此 Translocation leads to the occurrence and development of epithelial tumors, especially in the development of prostate cancer has a very important role. In order to better understand the role of fusion gene transcripts and to elucidate the clonal composition of multifocal tumors, the authors invented a technique for multichannel in situ detection and identification of fusion gene transcripts. Compared with immunohistochemistry TMPRSS2-ERG fusion negative and fusion positive prostate tumors. In human prostate cancer tissues, the authors identified and quantified the most prevalent TMPRSS2-ERG fusion variants and, for the first time, directly measured the proportion of different gene fusion transcripts by in situ sequencing. this