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用LKB－2107型微量热系统，在不同的温度（pH＝7．4）条件下，测定了3，4，5－三羟基苯甲酸与漆酶反应的摩尔反应烙、米氏常数、反应速率常数、漆酶的活性并计算了结合能、活化自由能、活化能和活化烟等．在此基础上，应用过渡态理论，从能量变化的角度，对其催化过程进行了分析．由活化摘（△S＜0）得出酶－底物过渡态的结构较酶－底物复合物更为有序的结论．
The molar reaction of 3,4,5-trihydroxybenzoic acid with laccase, the Michaelis constant, the reaction rate, and the reaction rate were measured at different temperatures (pH = 7.4) using the LKB-2107 micro calorimeter system Constants, laccase activity and calculated binding energy, activation energy, activation energy and activated tobacco. On this basis, the transition state theory is applied to analyze its catalytic process from the perspective of energy change. The result of activation digestion (△ S <0) shows that the structure of the enzyme-substrate transition state is more ordered than the enzyme-substrate complex.