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目的:探讨miR-638在肺腺癌中的表达及其与肺腺癌细胞凋亡的关系。方法:应用real-time RT-PCR的方法检测了miR-638在肺腺癌细胞株SPC-A1中的表达情况,并采用脂质体法将miR-638模拟物瞬时转染入SPC-A1。实验设置空白对照组、无关miRNA阴性对照组和miR-638转染组,转染后在荧光显微镜下观察转染效率,实时荧光定量RT-PCR检测miR-638的表达,流式细胞术检测各组细胞凋亡率。结果:与正常细胞相比,miR-638在肺腺癌细胞中低表达。SPC-A1细胞转染miR-638模拟物后,细胞凋亡率相对于空白组和阴性对照组显著升高(P<0.05)。结论:miR-638在肺腺癌中低表达,且能够促进肺腺癌细胞凋亡,可作为后续肺癌生物治疗的新分子靶标。
Objective: To investigate the expression of miR-638 in lung adenocarcinoma and its relationship with apoptosis of lung adenocarcinoma. Methods: Real-time RT-PCR was used to detect the expression of miR-638 in SPC-A1 cells. The miR-638 mimics were transiently transfected into SPC-A1 cells by liposome. The experiment set the blank control group, irrelevant miRNA negative control group and miR-638 transfection group, transfection efficiency was observed under a fluorescence microscope transfection efficiency, real-time fluorescent quantitative RT-PCR detection of miR-638 expression, flow cytometry Group apoptosis rate. Results: Compared with normal cells, miR-638 was low expressed in lung adenocarcinoma cells. The apoptosis rate of SPC-A1 cells transfected with miR-638 mimics was significantly higher than that of blank control group and negative control group (P <0.05). Conclusion: miR-638 is low expressed in lung adenocarcinoma and can promote the apoptosis of lung adenocarcinoma cells, which may serve as a new molecular target for the subsequent biological treatment of lung cancer.