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建立了以万古霉素为手性分离选择剂的毛细管电泳氨基酸对映体快速分离的方法.向缓冲溶液中加入0.002%(W/V)的(1,5-二甲基-1,5-二氮杂十一烷亚甲基聚N-甲溴化物(hexadimethrine bromide HDB)做电渗流改性剂,使电渗流的迁移方向反转,与分析物方向一致,缩短了分析时间.此外,HDB还使毛细管壁得到动态修饰,减少了万古霉素在毛细管壁上的吸附,从而提高了柱效.考察了缓冲溶液pH值、万古霉素浓度对分离的影响.在pH=6,分离电压为-18kV,万古霉素浓度为2mmol/L的条件下,对18种氨基酸对映体的9-芴基甲基甲酰氯衍生物进行了分离,有13种可以得到基线分离.平均分离柱效为3.2×10~5塔板每米.
A method for the rapid separation of enantiomers of capillary electrophoresis amino acids using vancomycin as a chiral separation selective agent was established.A solution of 0.002% (W / V) (1,5-dimethyl-1,5- The diazadunnylmethylene poly-N-methyl bromide (HBdimethrine bromide HDB) is an electroosmotic modifier, reversing the migration direction of the electroosmotic flow and keeping the direction of the analyte shortened the analysis time.In addition, the HDB The capillary wall was also dynamically modified to reduce the adsorption of vancomycin on the capillary wall, thereby increasing the column efficiency.Effects of buffer solution pH and vancomycin concentration on the separation were investigated.When pH = 6, the separation voltage was -18kV and vancomycin at the concentration of 2mmol / L, 18 kinds of amino acid enantiomers of 9-fluorenylmethyloyl chloride derivatives were isolated, 13 kinds can be baseline separation.Average column separation efficiency 3.2 × 10 ~ 5 plates per meter.