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目的为了探究1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)是否可引起小鼠体内Th17细胞因子IL-17和IL-23的表达变化。方法对8周龄C57BL/6J小鼠腹腔注射MPTP或PBS,于第0,1,2,5,7天进行前爪伸屈试验。并第8天处死后收集脑组织内纹状体,并检测血清中炎症因子的含量。结果实验结果表明,较PBS处理组,MPTP处理组雌、雄鼠的伸爪频率下降,并在处理后第1天有显著性差异,且在第2~7天的伸爪频率并没有改善,提示MPTP对于伸爪能力有抑制作用。在雄性小鼠中,经过MPTP处理后,IL-17在纹状体内的含量达到14900±330 ng/mg蛋白(对照组为12420±914 ng/mg蛋白),在血清的含量达到1970±145 ng/ml(对照组为1400±132 ng/ml)。然而雌性小鼠中未见IL-17的差异。IL-23在MPTP处理后的小鼠纹状体和血清中未见差异。结论本实验发现MPTP处理后的小鼠纹状体和血清中IL-17的表达上升,而IL-23未见明显变化,提示IL-17可能在小鼠PD模型中具有一定的作用。
Objective To investigate whether 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) can induce the expression of Th17 cytokines IL-17 and IL-23 in mice. Methods 8-week-old C57BL / 6J mice were injected intraperitoneally with MPTP or PBS, and flexion and flexion tests of forepaws were performed on days 0, 1, 2, 5, After sacrificed on the 8th day, the striatum in brain tissue was collected and the content of inflammatory cytokines in serum was detected. Results The experimental results showed that compared with the PBS group, the frequency of extensor claw decreased in female and male rats treated with MPTP, and there was a significant difference on the first day after treatment. The frequency of extensor claw did not improve on days 2 to 7, Tip MPTP inhibit the capacity of the claw. IL-17 levels in the striatum reached 14900 ± 330 ng / mg protein (12420 ± 914 ng / mg protein in the control group) after MPTP treatment in male mice and reached a level of 1970 ± 145 ng in the serum / ml (control group 1400 ± 132 ng / ml). However, no differences in IL-17 were found in female mice. IL-23 showed no difference in MPTP-treated mouse striatum and serum. Conclusion The results showed that the expression of IL-17 in the striatum and serum of MPTP-treated mice was increased, but there was no obvious change in IL-23, suggesting that IL-17 may play a role in the mouse PD model.