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目的研究电刺激迷走神经对肠缺血再灌注大鼠肝损伤的作用。方法30只成年雄性Wistar大鼠。行双侧颈部迷走神经切断术后,随机分为3组(n=10),对照组(C组):仅暴露腹腔;肠缺血再灌注组(II／R组):暴露腹腔后,夹闭肠系膜上动脉(SMA)1h,再灌注2 h;迷走神经刺激+肠缺血再灌注组(VNS组):在夹闭SMA前及再灌注开始时分别以5V、2ms和1 Hz强度的电能持续刺激左颈部迷走神经远端20 min;颈动脉插管以监测平均动脉压(MAP)。再灌注2h时处死大鼠,进行肝组织病变程度评价,测定肝组织肿瘤坏死因子-α(TNF-α)、核转录因子-κB(NF-κB)的表达以及血浆TNF-α浓度。结果与C组比较,II／R组再灌注期MAP下降,血浆TNF-α及肝组织TNF-α、NF-κB水平升高,肝组织病变程度加重(P<0．05或0．01);电刺激迷走神经可减弱上述改变。结论电刺激迷走神经可减轻肠缺血再灌注后肝损伤。
Objective To investigate the effect of electrical stimulation of vagus nerve on hepatic injury induced by intestinal ischemia-reperfusion in rats. Methods Thirty adult male Wistar rats. After bilateral cervical vagotomy, the rabbits were randomly divided into three groups (n = 10), the control group (group C): only the abdominal cavity was exposed; the intestinal ischemia / reperfusion group (II / R group) (SMA) for 1 h, and reperfusion for 2 h. VNS + VNS group: the electrical energy of 5 V, 2 ms and 1 Hz were sustained before and at the beginning of reperfusion The distal left vagal nerve was stimulated for 20 min; the carotid artery was cannulated to monitor mean arterial pressure (MAP). The rats were sacrificed at 2 hours after reperfusion. The degree of liver lesions was evaluated. The expression of TNF-α, NF-κB and TNF-α in plasma were measured. Results Compared with group C, the reperfusion MAP decreased, the levels of plasma TNF-α and TNF-α, NF-κB in liver tissue increased, and the severity of liver lesions increased in II / R group (P <0.05 or 0.01) Electrical stimulation of the vagus nerve attenuated these changes. Conclusion Electrical stimulation of vagus nerve can reduce hepatic injury after intestinal ischemia reperfusion.