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目的通过 MRI 研究大鼠肾上腺髓质增生(AMH)的信号特征,并与病理结果对比,探讨 MRI 诊断大鼠 AMH 的可能性。方法雄性 SD 大鼠60只,依体重大小,采取随机排列表法随机分为 A、B、C 实验组,皮下注射利血平0.4 mg·kg~(-1)·d~(-1);a、b、c 对照组,皮下注射等量生理盐水,每组10只。A、a,B、b,C、c 组分别连续注射40、60和80 d,行肾上腺 MR 扫描,并和病理研究作对照。结果注射60 d 后 B 组髓质百分数(34.3±5.8)%高于 b 组(25.7±8.9)%(t=2.462,P<0.05),C 组(36.4±7.8)%也高于 c 组(24.8±7.9)%(t=3.104,P<0.01),利血平注射60 d 后 AMH 造模成功;MR 下 B 和 b 组、C 和 c 组肾上腺体积分别为(50.7±14.1)和(41.1±11.0)、(52.7±10.5)和(40.4±10.7)mm~3,差异有统计学意义(t 值分别为2.352、3.442,P 值分别<0.05、<0.01);MR 下中心髓质区 T_2WI 信号 B 组高于 b 组(Z=-3.786,P<0.01)、C 组高于 c 组(Z=-3.570,P<0.01);光学显微镜(简称光镜)下见 B 和 C 组髓质明显增厚,细胞数增多、密集,呈实质性片状或条索状分布,形成细胞巢;实验组部分大鼠 T_1WI 下外周皮质信号明显增高,光镜下见束状带增生,细胞呈空泡状肥大,富含脂质。结论 MRT_2WI 中心髓质区信号增高及 T_1WI 外周皮质高信号可能对于诊断大鼠肾上腺髓质增生有意义。
Objective To investigate the signal characteristics of rat adrenal medullary hyperplasia (AMH) by MRI and to compare with the pathological results to explore the possibility of MRI in the diagnosis of AMH in rats. Methods Sixty male Sprague-Dawley rats were randomly divided into A, B, and C groups according to body weight, and were randomly divided into three groups: subcutaneous injection of reserpine 0.4 mg · kg -1 · d -1; A, b, c control group, subcutaneous injection of saline, each group of 10. A, a, B, b, C, c groups were injected continuously for 40, 60, and 80 d respectively. The adrenal MR scan was performed and compared with the pathological study. Results The percentage of medulla in group B (34.3 ± 5.8)% after 60 days of injection was significantly higher than that in group b (25.7 ± 8.9)% (t = 2.462, P <0.05) 24.8 ± 7.9)% (t = 3.104, P <0.01). The AMH model was successfully established after reserpine injection for 60 days. The volume of adrenal glands in groups B and b, C and c were (50.7 ± 14.1) and (T = 2.352, 3.442, respectively, P <0.05, <0.01, respectively). There was no significant difference between the two groups Signal B group was higher than group b (Z = -3.786, P <0.01), C group was higher than group c (Z = -3.570, P <0.01) Significantly thickening, the number of cells increased, dense, was a substantial sheet or bar-like distribution, the formation of cell nests; experimental group of rats peripheral T_1WI peripheral cortex signal was significantly increased, under light microscopy bundles hyperplasia, cells were empty Bubble hypertrophy, rich in lipids. Conclusion MRT_2WI center medullary region signal and peripheral T_1WI high signal may be useful for the diagnosis of adrenal medulla hyperplasia.