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目的研究人胎盘滋养细胞分离纯化培养及人乳头瘤病毒6型(HPV6)感染后其生物学特性的变化。方法用酶消化法和Percoll密度梯度离心分离培养人胎盘滋养细胞,免疫细胞组化染色鉴定其纯度,以HPV6感染后检测滋养细胞凋亡率。结果获得纯化的滋养细胞,HPV6感染48h后检测其凋亡率为(38.26±6.38)%。结论人乳头瘤病毒6型能感染滋养细胞并诱导其凋亡。
Objective To study the changes of biological characteristics of human placental trophoblast cells after isolation, purification and culture of human papillomavirus type 6 (HPV6). Methods Human placental trophoblast cells were isolated by enzyme digestion and Percoll density gradient centrifugation. Immunocytochemical staining was used to identify the purity of human trophoblast cells. The apoptosis rate of trophoblast cells was detected by HPV6 infection. Results The purified trophoblast cells were obtained. The apoptosis rate of HPV6 infected cells was (38.26 ± 6.38)% 48h after infection. Conclusion Human papillomavirus type 6 can infect trophoblasts and induce apoptosis.