将一个启动子序列与一个易检测的报告基因相连是研究转染真核细胞基因的表达的常用方法。这样报告基因如 :萤火虫荧光素酶 ,大肠杆菌氯霉素乙酰转移酶或大肠杆菌 β-半乳糖甘酶。为了减少细胞培养产生的变异 ,就蛋白浓度或细胞数目而言 ,各个加样孔的结果必须是标准化的。同时 Linking a promoter sequence to a reporter gene that is readily detectable is a common method of studying the expression of genes that have been transfected into eukaryotic cells. Such reporter genes are, for example, firefly luciferase, E. coli chloramphenicol acetyltransferase, or E. coli beta-galactosidase. In order to reduce the variation produced by cell culture, the results of individual wells must be standardized in terms of protein concentration or number of cells. Simultaneously