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核糖体蛋白是核糖体的主要组成成分,在细胞内蛋白质生物合成过程中具有重要作用。RPL29-1是核糖体大亚基60S的一个组件。本研究以花生品种日花1号为材料,利用RACE方法成功得到RPL29-1基因的c DNA序列,并获得DNA序列。RPL29-1基因c DNA序列包括186 bp的开放阅读框,编码61个氨基酸。RPL29-1基因DNA序列含有两个外显子和一个内含子。一级结构分析表明,RPL29-1蛋白质分子量为7 127.02 Da,等电点为10.29。本研究采用荧光定量技术(q RT-PCR)分析了RPL29-1基因在花生品种日花1号植株中的组织表达和青枯菌胁迫条件下的表达变化规律,并构建了RPL29-1的过表达载体和反义表达载体,为进一步通过功能分析研究花生RPL29-1基因在抗青枯病中的作用奠定基础。
Ribosomal protein is a major component of the ribosome and plays an important role in intracellular protein biosynthesis. RPL29-1 is a component of the 60S ribosomal large subunit. In this study, the peanut variety Rihua 1 was used as the material, and the cDNA sequence of RPL29-1 gene was obtained by RACE method. The RPL29-1 gene cDNA sequence contains a 186 bp open reading frame and encodes 61 amino acids. The RPL29-1 gene DNA sequence contains two exons and one intron. Primary structure analysis showed that the molecular weight of RPL29-1 protein was 7 127.02 Da and the isoelectric point was 10.29. In this study, RPL29-1 gene was analyzed by quantitative RT-PCR (q RT-PCR) analysis of tissue expression of RPL29-1 gene under the strain of Ralstonia solanacearum R% Expression vector and antisense expression vector, which laid the foundation for the further study on the function of peanut RPL29-1 gene in resistance to bacterial wilt through functional analysis.