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目的应用四氯化碳(CCl4)皮下注射方法,建立兔肝硬化动物模型。方法 50只雄性新西兰大白兔随机分为实验组(n=35)和对照组(n=15)。实验组臀部皮下注射50%CCl4橄榄油0.23 mL/kg,每周2次,对照组注射等量橄榄油。分别于4 w、8 w和12 w末检测各组兔肝脏功能及组织病理学变化。结果随时间延长,实验组兔肝脏纤维化程度逐渐加重,血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和γ-谷氨酰转肽酶(GGT)明显升高,12 w末出现白蛋白/球蛋白比例倒置,可观察到典型肝硬化病理表现,兔肝组织出现典型的假小叶结构,动物死亡率20%,造模成功率83%。对照组肝脏功能正常,肝脏组织学结构正常。结论此方法可成功地建立兔肝硬化动物模型,该方法成功率高,可用于相关实验研究。
Objective To establish an animal model of hepatic cirrhosis by subcutaneous injection of carbon tetrachloride (CCl4). Methods Fifty male New Zealand white rabbits were randomly divided into experimental group (n = 35) and control group (n = 15). The experimental group was subcutaneously injected 50% CCl4 olive oil 0.23 mL / kg in the buttocks twice a week. The control group received the same amount of olive oil. The liver function and histopathological changes of rabbits in each group were detected at the end of 4 w, 8 w and 12 w respectively. Results With the extension of time, hepatic fibrosis gradually increased in experimental group, serum ALT, AST and GGT increased significantly, and albumin appeared at the end of 12 w / Globulin ratio upside down, the pathological manifestation of typical cirrhosis was observed. The typical pseudolobule structure appeared in rabbit liver tissue. The animal mortality rate was 20% and the success rate of modeling was 83%. The control group had normal liver function and normal liver histology. Conclusion This method can successfully establish rabbit liver cirrhosis animal model. The method has high success rate and can be used in relevant experimental research.