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目的探讨子宫内膜异位症(内异症)患者腹腔液及血清中,前列腺素E2(PGE2)和bcl-2蛋白的含量变化及其与内异症发病的关系。方法采用酶联免疫吸附法(ELISA)检测36例内异症患者(内异症组,其中Ⅰ、Ⅱ期患者16例,Ⅲ、Ⅳ期患者20例)和30例因卵巢囊肿或浆膜下子宫肌瘤手术患者(对照组)腹腔液及血清PGE2与bcl-2含量。结果内异症组患者腹腔液中PGE2、bcl-2蛋白的含量分别为(1987±532)ng/L、(177±53)U/L,对照组为(386±215)ng/L、(86±21)U/L,两组比较,差异均有统计学意义(P<0.01)。Ⅲ、Ⅳ期内异症患者腹腔液中PGE2的含量[(2221±1352)ng/L]较Ⅰ、Ⅱ期患者[(1694±381)ng/L]明显升高,差异也有统计学意义(P<0.01);Ⅲ、Ⅳ期内异症患者腹腔液中bcl-2的含量[(190±63)U/L]较Ⅰ、Ⅱ期患者[(162±49)U/L]升高,差异有统计学意义(P<0.05)。内异症组血清中的PGE2与bcl-2含量[(3787±514)ng/L、(96±44)U/L]均高于对照组[(129±97)ng/L、(53±40)U/L],差异均有统计学意义(P<0.01)。Ⅲ、Ⅳ期内异症患者血清中的PGE2含量[(964±290)ng/L]也高于Ⅰ、Ⅱ期患者[(590±362)ng/L],差异也有统计学意义(P<0.01);内异症患者腹腔液中PGE2及bcl-2的含量均高于血清。结论内异症患者腹腔中高含量的PGE2与bcl-2,可能对内异症发病有影响;内异症患者腹腔液与血清中PGE2和bcl-2含量与病变的范围有一定关系。
Objective To investigate the changes of prostaglandin E2 (PGE2) and bcl-2 protein in peritoneal fluid and serum of patients with endometriosis (endometriosis) and its relationship with the pathogenesis of endometriosis. Methods Thirty - six patients with endometriosis (including 16 patients with stage Ⅰ and Ⅱ, 20 patients with stage Ⅲ and Ⅳ) and 30 patients with endometriosis due to ovarian cyst or subserosus were enrolled in this study. The contents of PGE2 and bcl-2 in peritoneal fluid and serum in patients with uterine fibroid surgery (control group). Results The levels of PGE2 and bcl-2 protein in peritoneal fluid of patients with endometriosis were (1987 ± 532) ng / L and (177 ± 53) U / L, respectively, and those in control group were (386 ± 215) ng / 86 ± 21) U / L, the differences between the two groups were statistically significant (P <0.01). The levels of PGE2 in peritoneal fluid of patients with endometriosis Ⅲ and Ⅳ [(2221 ± 1352) ng / L] were significantly higher than those in patients of stage Ⅰ and Ⅱ [(1694 ± 381) ng / L], and the difference was also statistically significant P <0.01). The content of bcl-2 in peritoneal fluid of patients with endometriosis in stage Ⅲ and Ⅳ [(190 ± 63) U / L] was higher than that in stage Ⅰ and Ⅱ [(162 ± 49) U / L] The difference was statistically significant (P <0.05). The levels of PGE2 and bcl-2 in the endometriosis group were significantly higher than those in the control group [(3787 ± 514) ng / L, (96 ± 44) U / L] 40) U / L], the differences were statistically significant (P <0.01). The level of PGE2 in patients with stage III and IV endometriosis was also significantly higher than that in stage I and II [(590 ± 362) ng / L, P < 0.01). The contents of PGE2 and bcl-2 in peritoneal fluid of patients with endometriosis were higher than those of serum. Conclusions The high content of PGE2 and bcl-2 in the peritoneal cavity of endometriosis patients may have an impact on the pathogenesis of endometriosis. The content of PGE2 and bcl-2 in peritoneal fluid and serum of patients with endometriosis has a certain relationship with the extent of the disease.