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目的研究广东省人免疫缺陷病毒1(HIV-1)亚型的基因序列特征。方法 2002年采集100份 HIV-1感染者的外周静脉抗凝血,分离单核细胞(PBMC),提取前病毒 DNA,通过聚合酶链反应(PCR)扩增,获得包膜蛋白(ENV)区基因的核酸片段,并对其 C2～V3及邻区核苷酸序列进行测定和分析。所得序列用 GCG 软件包进行系统进化树和氨基酸变异分析。结果经过离散率计算和系统进化树分析后证实,其 PCR 扩增阳性的75份样品中,44份(58.7%)为重组毒株 CRF01-AE(circulating recombinant form 01-AE),27份(36%)为 CRF-BC,4份为泰国 B′亚型(5.3%)。结论广东省 HIV-1以 CRF01-AE 和 CRF-BC 为主,也存在泰国B′亚型。目前我省境内有多种亚型存在,流行趋势日益严峻。加强艾滋病患者的治疗,以及控制和预防艾滋病的传播是今后面临的重要任务。
Objective To study the gene sequence of human immunodeficiency virus 1 (HIV-1) in Guangdong Province. Methods A total of 100 peripheral blood mononuclear cells (PBMCs) were collected from HIV-1 infected patients in 2002. The proviral DNA was extracted and amplified by polymerase chain reaction (PCR) Gene of nucleic acid fragments, and its C2 ~ V3 and adjacent nucleotide sequence determination and analysis. The obtained sequence was analyzed by phylogenetic tree and amino acid variation using GCG software package. The results of discrete rate calculation and phylogenetic tree analysis confirmed that among the 75 samples positive for PCR amplification, 44 (58.7%) were recombinant clot-form 01-AE (CRF01-AE), 27 %) For CRF-BC and 4 for Thai B 'subtype (5.3%). Conclusion HIV-1 in Guangdong province mainly contains CRF01-AE and CRF-BC, but also Thailand B 'subtype. At present, there are many subtypes in our province, and the epidemic trend is becoming more and more serious. Strengthening the treatment of AIDS patients and controlling and preventing the spread of AIDS are important tasks to be solved in the future.