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目的:建立同时测定中药远志中有效成分远志酮Ⅲ和3,6’-二芥子酰基蔗糖的HPLC含量测定方法。方法:采用Kromasil C18(250mm×4.6mm,5μm)色谱柱,以乙腈-0.05%磷酸水溶液(18:82)为流动相,流速1.0mL.min-1,检测波长320nm,柱温30℃。结果:远志酮Ⅲ及3,6’-二芥子酰基蔗糖的线性范围分别为0.5～4.0μg(r=0.9996)和0.5～8.0μg(r=0.9996),其平均回收率(n=6)分别为97.1%(RSD=0.87%)和99.4%(RSD=1.5%)。结论:该方法准确,重复性好,能够同时对远志中2种有效成分进行含量测定,为远志药材的质量控制提供了有效的方法。
OBJECTIVE: To establish a method for the simultaneous determination of HPLC of far-endone III and 3,6’-alanylsucrose in Polygala tenuifolia. Methods: The mobile phase was acetonitrile-0.05% phosphoric acid solution (18:82) with a flow rate of 1.0 mL · min-1 on a Kromasil C18 column (250 mm × 4.6 mm, 5 μm) with a detection wavelength of 320 nm and a column temperature of 30 ° C. Results: The linear ranges of farnesyl ketone Ⅲ and 3,6’-alanyl sucrose were 0.5 ~ 4.0μg (r = 0.9996) and 0.5 ~ 8.0μg (r = 0.9996) 97.1% (RSD = 0.87%) and 99.4% (RSD = 1.5%), respectively. Conclusion: The method is accurate and reproducible. It can simultaneously determine the content of two active ingredients in Polygala tenuifolia and provide an effective method for the quality control of Polygala tenuifolia.