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目的:测定消癌平注射液中新绿原酸、原儿茶醛、绿原酸、隐绿原酸、咖啡酸、香草酸和4-香豆素的含量。方法:Kromasil 100-5 C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.2%磷酸,梯度洗脱,检测波长300 nm;柱温30℃,流速0.8mL·min-1。结果:新绿原酸、原儿茶醛、绿原酸、隐绿原酸、咖啡酸、香草酸和4-香豆酸的线性范围分别为0.1~3.2 mg·L-1(r=0.999 3)、0.025~0.8 mg·L-1(r=0.999 9),0.1~3.2 mg·L-1(r=0.999 8),0.075~2.4 mg·L-1(r=0.999 9),0.037 5~1.2 mg·L-1(r=0.999 9),0.02~0.64 mg·L-1(r=0.999 9),0.01~0.32 mg·L-1(r=0.999 9),加样回收率分别为99.6%(RSD 0.24%),100.0%(RSD 0.15%),98.7%(RSD 1.00%),99.1%(RSD 1.36%),96.4%(RSD 1.37%),98.3%(RSD1.80%),97.3%(RSD 1.64%)。结论:该方法简便,准确,重复性好,可为消癌平注射液提供质量控制依据。
Objective: To determine the contents of neogenvate, protocatechuic aldehyde, chlorogenic acid, cryptocholic acid, caffeic acid, vanillic acid and 4-coumarin in Xiaojianping injection. METHODS: Kromasil 100-5 C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was eluted with acetonitrile-0.2% phosphoric acid. The detection wavelength was 300 nm. The column temperature was 30 ℃ and the flow rate was 0.8 mL · min-1. Results: The linear ranges of neogenuric acid, protocatechuic aldehyde, chlorogenic acid, cryptocholic acid, caffeic acid, vanillic acid and 4-coumaric acid were 0.1-3.2 mg · L -1 (r = 0.999 3) , 0.025-0.8 mg · L-1 (r = 0.999 9), 0.1-3.2 mg · L-1 (r = 0.999 8), 0.075-2.4 mg · L -1 (r = 0.999 9) (r = 0.999 9), 0.02-0.64 mg · L-1 (r = 0.999 9) and 0.01-0.32 mg · L-1 (r = 0.999 9) (RSD 0.24%), 100.0% RSD 0.15%, 98.7% RSD 1.00%, 99.1% RSD 1.36%, 96.4% RSD 1.37%, 98.3% RSD 1.80%, 97.3% RSD 1.64%). Conclusion: The method is simple, accurate, reproducible, and can provide quality control basis for Xiao-Jin-Ping injection.