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目的 研究我国多发性硬化 (MS)病人外周血单个核细胞 (PBMC)转录因子Sp3基因表达情况及其与临床相关性。方法 采用反转录 聚合酶链反应 (RT PCR)技术 ,经 2对引物扩增 ,检测了 33例MS、30例非免疫性其他神经系统疾病对照、30例神经系统其他自身免疫性疾病对照和 30名健康对照者PBMC的Sp3基因表达。结果 Sp3基因表达缺如 ,MS患者组 (45 % ,15 / 33)明显高于其他 3组 (依次为 16 % ,5 / 30 ;6 % ,2 / 30 ;10 % ,3/ 30 ;P均 <0 .0 1)。可自Sp3表达阴性MS患者的DNA中扩增出相应Sp3片段。Sp3表达阴性MS患者改良的伤残状态量表 (EDSS)评分显著高于表达阳性者。脑脊液的IgG 2 4h合成率和血清可溶性白细胞介素 2受体稍高。 4例MS患者治疗前病情重和治疗后病情轻时相比 ,未见其Sp3基因表达有明显变化。结论 汉人MS病人PBMC中有Sp3表达缺陷 ,Sp3阴性患者残疾程度和免疫功能紊乱程度明显高于阳性者 ,作为转录调节因子Sp3的表达缺乏可能与免疫控制的异常启动有关
Objective To investigate the expression of Sp3 gene in peripheral blood mononuclear cells (PBMCs) of patients with multiple sclerosis (MS) and its clinical significance. Methods Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify and detect 33 cases of MS, 30 cases of other non-immune system diseases and 30 cases of other autoimmune diseases of nervous system Sp3 gene expression in PBMC of 30 healthy controls. Results The expression of Sp3 gene was absent. The MS patients (45%, 15/33) were significantly higher than the other three groups (16%, 5/30, 6%, 2/30, 10%, 3/30, P <0 .0 1). The corresponding Sp3 fragments can be amplified from the DNA of Sp3-negative MS patients. The modified Disability Status Scale (EDSS) score for patients with Sp3-negative MS was significantly higher than those with positive expression. Cerebrospinal fluid IgG 2 4h synthesis rate and serum soluble interleukin 2 receptor slightly higher. There was no obvious change of Sp3 gene expression in 4 MS patients before treatment, compared with their mild condition after treatment. Conclusion The expression of Sp3 in PBMC of Chinese MS patients is defective. The degree of disability and immune dysfunction in Sp3-negative patients is significantly higher than that of positive ones. The lack of expression of Sp3 as a transcriptional regulator may be related to the abnormal activation of immune control