论文部分内容阅读
目的:探讨唑来膦酸(Zol)联合IL-2对健康人和骨肉瘤患者末梢血γδT细胞的扩增和细胞毒性作用的影响。方法:取健康人和骨肉瘤患者外周血来源的单个核细胞,分别使用IL-2和Zol联合IL-2刺激体外扩增,流式细胞技术检测γδT细胞的纯度,并计算其扩增倍数。以Daudi细胞作为阳性对照、Raji细胞为阴性对照,并以人成骨细胞系hFOB细胞作为正常对照,用LDH法检测扩增后γδT细胞的细胞毒活性的变化。结果:健康人和骨肉瘤患者外周血单核细胞经过体外14 d的培养,Zol联合IL-2组可高度选择性扩增γδT细胞,并且扩增后γδT细胞具有杀伤活性,而对正常细胞无杀伤活性。结论:健康人和骨肉瘤患者PBMCs经唑来膦酸联合IL-2刺激后,可获得高纯度具有较强的杀伤作用的γδT细胞。
Objective: To investigate the effects of Zol combined with IL-2 on the proliferation and cytotoxicity of γδT cells in peripheral blood of healthy human and osteosarcoma patients. Methods: Peripheral blood mononuclear cells were obtained from healthy volunteers and osteosarcoma patients. IL-2 and Zol combined with IL-2 were respectively used to stimulate in vitro expansion. The purity of γδT cells was detected by flow cytometry and the amplification factor was calculated. Daudi cells as a positive control, Raji cells as a negative control, and human osteoblast cell line hFOB cells as a normal control, using the LDH method to detect the expansion of γδ T cells cytotoxic activity changes. RESULTS: Peripheral blood mononuclear cells from healthy volunteers and osteosarcoma patients were cultured in vitro for 14 days. Zol combined with IL-2 group could highly selectively amplify γδT cells, and γδT cells had cytotoxic activity after amplification. However, normal cells Killing activity. CONCLUSIONS: PBMCs from healthy and osteosarcoma patients can be stimulated by zoledronic acid combined with IL-2 to obtain γδT cells with high purity and high cytotoxicity.