【摘 要】
：
Elevated activities of cytosolic fructose-1,6-bisphosphatase (cyFBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) are associated with higher yields in plants. In this study, the expression levels of the cyFBPase and SBPase genes were increased by over
【机 构】
：
Biotechnology Research Institute,Chinese Academy of Agricultural Sciences/National Key Facility for
论文部分内容阅读
Elevated activities of cytosolic fructose-1,6-bisphosphatase (cyFBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) are associated with higher yields in plants. In this study, the expression levels of the cyFBPase and SBPase genes were increased by overexpressing rape (Brassica napus) cDNA in tobacco (Nicotiana tabacum) plants. The transgenic plants co-expressing cyFBPase and SBPase (TpFS), or expressing single cyFBPase (TpF) or SBPase (TpS) had 1.77-, 1.55-, 1.23-fold cyFBPase and 1.45-, 1.12-, 1.36-fold SBPase activities as compared to the wild-type (WT), respectively. Photosynthesis rates of TpF, TpS and TpFS increased 4, 20 and 25％ compared with WT plants. The SBPase and cyFBPase positively regulated each other and functioned synergistically in transgenic tobacco plants. In addition, the sucrose contents of the three transgenic plants were higher than that of WT plants. The starch accumulation of the TpFS and TpS plants was improved by 53 and 37％, but slightly decreased in TpF plants. Moreover, the transgenic tobacco plants harbouring SBPase and/or cyFBPase genes showed improvements in their growth, biomass, dry weight, plant height, stem diameter, leaf size, flower number, and pod weight. In conclusion, co-expression of SBPase and cyFBPase may pave a new way for improving crop yield in agricultural applications.
其他文献
大学课程建设是高等教育内涵建设的核心,直接决定着高等教育人才培养质量的高低,打造“金课”,已成为当前中国大学课程与教学改革的热点问题.在新农科建设背景下,本文通过对农学动物医学专业《兽医临床诊断学》核心课程目前教学改革的现状、主要面临的问题及如何打造“金课”进行探讨,为一流本科课程建设提供一定的参考.
Castration of male animals is a common practice in the meat industry aimed at reducing aggressive behavior, preventing unpleasant flavor, and controlling undesirable breeding. For many years, mechanical castration and surgical castration have been practic
China has the world\'s largest sheep flock and the largest producer of sheep meat by far. However, the nutrient requirements have not been systemically studied and there is a lack of knowledge of nutrient requirement for meat-type sheep in China. Since
Nitrate reductase (NR) is an important enzyme for nitrate assimilation in plants, and post-translational phosphorylation regulates NR activity. To evaluate the impact of the dephosphorylation of nitrate reductase 1 (NIA1) protein on NR activity, nitrogen
The plant hydraulic network faces several challenges under drought stress. Hydraulic conductivity is one of the major indicators of the hydraulic network\'s response to drought stress. Here, we review our current understanding of the factors directly af
Soybean (Glycine max (L.) Merr.), a typical short-day plant, is sensitive to photoperiod, which limits the geographical range for its cultivation. In the flowering pathway regulated by photoperiod, E1, a flowering inhibitor in soybean, plays the dominant
With the application of hybrid wheat, lodging is becoming one of the major factors limiting high yield in its production. However, few studies have focused on combining ability and heterosis analysis of stem-related traits. In this study, 24 crosses were
The introduction of genetically modified (GM) soybean into farming systems raises great concern that transgenes from GM soybean may flow to endemic wild soybean via pollen. This may increase the weediness of transgenic soybean by increasing the fitness of
The discovery and application of environment-sensitive genic male sterile (EGMS) rice germplasm provide an easy method for hybrid rice breeding and have made great contributions to hybrid rice production. Typically, the photoperiod- and thermo-sensitive G
Excessive rainfall provides a favorable condition for field mold infection of plants, which triggers field mold (FM) stress. If FM stress occurs during the late maturation stage of soybean seed, it negatively affects seed yield and quality. To investigate