酶组织化学定量大都是静态的研究,即在同一温度、时间、pH值和底物浓度条件下,比较组织化学反应产物的灰度(深浅)进行定量,从而反映酶活性的强弱。用组织化学方法研究酶动力学来反映酶活性的工作并不多。本实验采用大鼠肾脏新鲜冰冻连续切片,厚6μm。对相邻切片进行不同时间(3、5、7、11、20和30分钟)、不同的pH值(pH7、8、9、9.4、10和11)和不同底物 Enzyme histochemical quantification is mostly a static study, that is, at the same temperature, time, pH and substrate concentration conditions, compared the histochemical reaction product of the gray (shades) to quantify, reflecting the strength of the enzyme activity. There are not many efforts to study enzyme kinetics to reflect enzyme activity using histochemical methods. In this experiment, rat kidney fresh frozen serial slices, thickness 6μm. The adjacent sections were exposed to different times (3, 5, 7, 11, 20 and 30 minutes), different pH values ​​(pH 7, 8, 9, 9, 4, 10 and 11) and different substrates