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目的:建立测定小儿感冒宁颗粒中牛蒡苷含量的高效液相色谱法。方法:采用Dikma diamonsil C_(18)色谱柱(250mm×4.6mm,5μm),流动相:乙腈-水(25:75),流速:1.0ml·min~(-1),检测波长:280nm。结果:牛蒡苷在0.558~11.156μg范围内(r=0.9997)线性关系良好,平均回收率为96.2%(RSD=0.7%)。结论:该方法简便、灵敏、准确,可用于该制剂的质量控制。
Objective: To establish a HPLC method for the determination of the content of arctiin in Xiaoerganning granules. Methods: Dikma diamonsil C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and water (25:75). The flow rate was 1.0 ml · min -1. The detection wavelength was 280 nm. RESULTS: Arctiin had a good linear relationship in the range of 0.558-11.156 μg (r = 0.9997) with an average recovery of 96.2% (RSD = 0.7%). Conclusion: The method is simple, sensitive and accurate and can be used for the quality control of the preparation.