论文部分内容阅读
目的 :观察造血生长因子对受照射小鼠辐射损伤的防治作用。方法 :用大鼠浆细胞株培养上清 (RSP- CM)和小鼠成纤维细胞株培养上清 (L P3- CM)来源的集落刺激因子 ,治疗受不同剂量 6 0 Coγ射线照射的 ICR小鼠。观察小鼠 30 d存活率、死亡动物平均存活日、保护系数及部分动物外周血白细胞计数。研究 GM- CSF对受照射小鼠重建造血功能的疗效及量效、时效关系。结果 :RSP- CM能显著提高受 7.5 Gy照射小鼠的存活率 ,而 L P3- CM则无效。照射剂量为 8.0 Gy时 ,只有 RSP- CM治疗组有效 ,预防组和防治组无效。照射剂量为 8.5 Gy时 ,小鼠 30 d内全部死亡 ,表明 GM- CSF对大剂量照射小鼠疗效差 ,但从保护系数看 ,各实验组均有效 ,且存在量效、时效关系。结论 :RSP- CM源性 GM- CSF对受照小鼠的骨髓损伤有促进造血恢复的作用 ,且在一定程度上存在量效、时效关系 ,而 L P3- CM源性 M- CSF则无效。
Objective: To observe the preventive and therapeutic effects of hematopoietic growth factor on radiation injury in irradiated mice. Methods: The colony-stimulating factor derived from rat plasma cell culture supernatant (RSP-CM) and mouse fibroblast cell culture supernatant (L P3-CM) was used to treat ICRs exposed to different doses of 60 Coγ-rays mouse. The survival rate of mice at 30 d, the average survival days of the dead animals, the protection coefficient and the peripheral blood leukocyte counts of some animals were observed. To study the curative effect and dose-effect and time-effect relationship of GM-CSF on the hematopoiesis in irradiated mice. RESULTS: RSP-CM significantly increased the survival of mice exposed to 7.5 Gy, whereas L P3-CM did not. At the dose of 8.0 Gy, only the RSP-CM treatment group was effective and the prevention and control groups were ineffective. The irradiation dose of 8.5 Gy all mice died within 30 d, indicating that GM-CSF on high-dose irradiated mice curative effect is poor, but from the protection factor, the experimental group were effective, and there is a dose-effect, time-effect relationship. CONCLUSION: RSP-CM-derived GM-CSF can promote hematopoietic recovery in irradiated mice, and there is dose-effect and time-effect relationship to a certain degree. L P3-CM-derived M-CSF is ineffective.