论文部分内容阅读
目的 采用油酸和内毒素先后两次致伤大鼠 ,建立两次打击急性肺损伤动物模型。方法 油酸 (0 .2ml/kg)经尾静脉注入 ,伤后 1、4、12和 2 4h给予小剂量大肠杆菌脂多糖 (LPS ,2mg/kg)再次打击。结合动脉血气分析、外周血白细胞计数与分类变化及肺组织病理观察 ,评估两次打击大鼠急性肺损伤模型。结果 油酸致伤 4h加LPS 2次打击大鼠 ,PaO2 降低、外周血白细胞数增多和肺组织损伤最严重 ,与对照组比较相差显著。结论 油酸加LPS两次打击大鼠急性肺损伤模型 ,能较好地反映油酸致伤后第二次小剂量内毒素打击引发严重肺损伤的病理变化。
OBJECTIVE To establish two animal models of acute lung injury by twice injuring rats with oleic acid and endotoxin. Methods Oleic acid (0.2ml / kg) was injected through the tail vein. Small doses of lipopolysaccharide (LPS, 2mg / kg) were administered to rats at 1, 4, 12 and 24 hours after injury. Combined with arterial blood gas analysis, peripheral blood leukocyte count and classification changes and lung histopathological observation, two-hit model of acute lung injury in rats was evaluated. Results oleic acid injury 4 h plus LPS twice hit rats, PaO2 decreased peripheral blood leukocyte count and lung tissue damage the most serious, compared with the control group, a significant difference. Conclusion Both oleic acid and LPS can effectively reflect the pathological changes of severe lung injury caused by the second dose of LPS after oleic acid injury in acute lung injury model.