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由于近年来各种抗生素在临床上广泛应用,致病菌对药物的耐药性逐年增加,影响抗生素的临床疗效。因此,抗生素的联合应用也被人们所重视,藉以防止或延缓耐药菌株的产生,提高抗生素的临床疗效,减少用量,降低毒副反应。 我们采用实验室的方法,用国产抗生素和抗菌增效剂(TMP)与创新霉素组合,观察对大肠杆菌的抑制作用和体内保护作用。本文仅就初步实验结果作简单小结。 材料和方法 1.菌株:试验菌株大肠杆菌6株,2株为实验室保存,4株为临床分离的菌株。保存在普通琼脂斜面上,放置冰箱备用。 2.抗生素:除创新霉素外,其他22个抗生素和抗菌增效剂(TMP),大部份由药品生物制品检定所供给标准品,小部份为市售商品。按理化性质,用有机溶媒或无盐水溶解,分别用肉膏汤稀释成适当浓度备用。
Due to the wide application of various antibiotics in clinical practice in recent years, the drug resistance of pathogens increases year by year, affecting the clinical efficacy of antibiotics. Therefore, the joint application of antibiotics has also been people’s attention, in order to prevent or delay the generation of drug-resistant strains, improve the clinical efficacy of antibiotics, reduce dosage and reduce side effects. We use the laboratory method, with domestic antibiotics and antibacterial synergist (TMP) and the combination of innovative to observe the inhibition of E. coli and in vivo protective effect. This article only a brief summary of preliminary experimental results. MATERIALS AND METHODS 1. Strain: Six strains of E. coli were tested, two were kept in laboratory and four strains were clinically isolated. Store on an ordinary agar slant, place the refrigerator spare. 2. Antibiotics: In addition to neomycin, the other 22 antibiotics and antimicrobial synergists (TMP), most of the standards provided by the pharmaceutical and biological products test, a small part of the commercial products. According to physical and chemical properties, dissolved in organic solvent or no salt water, respectively, diluted with meat extract soup into the appropriate concentration for use.