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通过水传播病源是发展中国家最常见的疾病传播因素。通常是由于缺乏有保护的水源供应或者是供水系统出了毛病而造成的。常规用于检查水质量的细菌学试验是测定每100毫升水样中大肠杆菌的最大可能值(MPN)。要获得这种试验的结果,需要有合格的技术人员、一定的试验设备和72小时的时间。这对广泛地实施水质污染检查,特别就农村来讲是有很大困难的。作者们观察到,饮用水中大肠杆菌的存在始终是与产生H_2S的微生物相关联的,而且有些肠道细菌,如沙门氏菌属和变形杆菌属等也产生H_2S。根据这一机理,作者设计了一种检测饮用水粪便污染的简便现场试验方法。这项试验需要一种培养基,它由20克胨,15克磷酸氢二钾,0.75克枸橼酸铁,1
The source of transmission through water is the most common disease transmission factor in developing countries. This is usually caused by a lack of a protected water supply or by a faulty water supply system. The bacteriological test routinely used to check the quality of water is to determine the maximum possible value (MPN) of E. coli per 100 ml of water sample. To obtain the result of this kind of test, need to have qualified technical personnel, certain test equipment and 72 hours of time. This is a great challenge to implementing a wide range of water quality inspections, especially in rural areas. The authors observed that the presence of E. coli in drinking water was always associated with H 2 S-producing microorganisms and that some intestinal bacteria, such as Salmonella and Proteus, also produced H 2 S. Based on this mechanism, the authors designed a simple on-site test method for detecting faecal contamination of drinking water. This test requires a culture medium consisting of 20 grams of peptone, 15 grams of dipotassium hydrogen phosphate, 0.75 grams of iron citrate, 1