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目的探讨端粒酶在病理性消化道息肉中的检测及在早期胃癌或大肠癌诊断中的价值。方法端粒重复序列扩增法(TRAP-PCR-PAGE)检测175例胃镜活检标本端粒酶活性,标本均系电子内镜下取得的病理性胃、肠息肉组织。结果增生性息肉、腺瘤、炎性息肉和息肉样胃(肠)癌组织的端粒酶阳性率分别为6.2%、19.5%、15.4%和85.7%;同时随着息肉直径的增大、数目的增多,其端粒酶的阳性表达率也逐渐增高。结论端粒酶活性检测可作为消化道息肉恶变的早期预测指标。
Objective To investigate the detection of telomerase in pathological gastrointestinal polyps and its value in the diagnosis of early gastric cancer or colorectal cancer. Methods The telomerase activity of 175 cases of gastroscopic biopsy specimens was detected by TRAP-PCR. The specimens were all obtained by electronic endoscopy. Results The positive rates of telomerase in proliferative polyps, adenomas, inflammatory polyp and polyposis stomach carcinoma were 6.2%, 19.5%, 15.4% and 85.7%, respectively. Meanwhile, with the increase of polyp diameter, The increase of telomerase positive expression rate also gradually increased. Conclusion The detection of telomerase activity can be used as an early predictor of malignant transformation of gastrointestinal polyps.