论文部分内容阅读
目的了解海南省不明原因发热病人中钩端螺旋体病的感染情况。方法分别用钩端螺旋体显微镜凝集试验(MAT)、酶联免疫试验(ELISA)和聚合酶链反应(PCR)法,检测海南省2015年全省不明原因发热病人的血标本。结果共检测标本179份,其中显微镜凝集试验检出阳性标本40份,感染率为22.35%,男性感染率为21.65%(21/97),女性感染率为23.17%(19/82);菌群中有7个群能检出,分别是问号钩端螺旋体黄疸出血群、拜伦群、秋季热群、波摩那群、流感伤寒群、赛罗群、塔拉索夫群;酶联免疫试验检测阳性38份,阳性率为21.23%,其中IgM阳性6份,检出率为3.35%,IgG阳性32份,检测率为17.88%;血清学检测结果一致。PCR法检测结果均为阴性。结论海南省不明原因发热病人中存在钩端螺旋体的感染,主要型别以黄疸出血群为主。
Objective To understand the infection of leptospirosis in patients with fever of unknown origin in Hainan Province. Methods Blood samples of patients with unexplained fever in Hainan province were detected by using the leptospirosis microscopy agglutination test (MAT), enzyme - linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Results A total of 179 specimens were detected. Among them, 40 specimens were positive by microscopic agglutination test, the infection rate was 22.35%, the male infection rate was 21.65% (21/97) and the female infection rate was 23.17% (19/82) Seven groups were detected, including Leptospira interrogans, Hepatic jaundice, Byron group, Fall heat group, Pomona group, Influenza typhoid group, Cyrillic group and Tarassov group, respectively. Enzyme-linked immunosorbent assay Positive 38, the positive rate was 21.23%, of which 6 were IgM positive, the detection rate was 3.35%, IgG positive 32, the detection rate was 17.88%; serological test results. PCR test results were negative. Conclusion There is Leptospira infection in patients with fever of unknown cause in Hainan Province. The main type is the hemorrhagic group with jaundice.