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目的探讨原花青素(proanthocyanidins,PC)对染尘大鼠肺纤维化进程的具体作用。方法 SPF级SD大鼠96只,随机分为对照组、矽肺组、PC低剂量组、PC高剂量组,每组24只;采用气管内注射SiO2悬液方法复制大鼠矽肺模型。染尘后第2天PC低剂量组按50 mg/(kg.d)灌胃给予PC,PC高剂量组按500 mg/(kg.d)灌胃给予PC,矽肺组和对照组则给予等容生理盐水。给药开始后第14、28、60天时每组随机处死8只大鼠;应用免疫组化法观察肺组织TGF-β1、HSP47的表达,并检测大鼠血清超氧化物歧化酶(SOD)活力。结果与对照组比较,大鼠在染尘14、28和60 d时,血清中SOD的活力明显下降,60 d时尤为显著;此外,肺组织中TGF-β1和HSP47的表达增加,且随染尘时间增加呈现上升趋势。应用高剂量PC干预后,血清中SOD活性增加(P<0.05),在染尘60 d时,上升到251.36 U/ml(矽肺组为181.32 U/ml);PC干预组肺组织中TGF-β1和HSP47表达的升高趋势(P<0.05)亦见恢复,染尘60 d时,PC高剂量组肺组织中TGF-β1和HSP47的表达分别为24.45和13.54(矽肺组为33.27和18.70)。结论原花青素可增强SOD活力,减少TGF-β1和HSP47的表达,提示可在一定程度上缓解染尘大鼠肺纤维化进程。
Objective To investigate the effect of proanthocyanidins (PC) on pulmonary fibrosis in rats exposed to dust. Methods Ninety SPF SD rats were randomly divided into control group, silicosis group, PC low dose group and PC high dose group, with 24 rats in each group. Silicosis model was induced by intratracheal injection of SiO2 suspension. On the second day after the dying, the PC low dose group was given intragastrically with PC (50 mg / (kg · d)), the PC high dose group was given intragastrically with PC (500 mg / (kg · d)), while the silicosis group and the control group Physiological saline. At the 14th, 28th, and 60th day after the start of administration, 8 rats were randomly sacrificed in each group. The expression of TGF-β1 and HSP47 in the lung tissue was detected by immunohistochemistry and the activity of superoxide dismutase (SOD) . Results Compared with the control group, the activity of SOD in serum decreased significantly at 14, 28 and 60 days after dying for dust, especially at 60 days. In addition, the expression of TGF-β1 and HSP47 in lung increased, Dust time increases showing an upward trend. The serum SOD activity increased (P <0.05) and increased to 251.36 U / ml (181.32 U / ml in the silicosis group) after 60 days of dying, while TGF-β1 (P <0.05). The expression of TGF-β1 and HSP47 in lung tissue of PC high-dose group were 24.45 and 13.54 respectively (33.27 and 18.70 in silicosis group). Conclusions Proanthocyanidins can enhance the activity of SOD and decrease the expression of TGF-β1 and HSP47, suggesting that it can alleviate the pulmonary fibrosis in rats exposed to dust in a certain extent.